GM-CSF antibody | 3209 (30-4)
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Mouse anti Human GM-CSF antibody, clone 3209 recognizes recombinant and native human granulocyte macrophage colony stimulating factor (GM-CSF).
Human Granulocyte colony stimulating factor (GM-CSF) is a 14.6 kDa glycoprotein, which is produced by T cells, macrophages, fibroblasts and endothelial cells. GM-CSF is involved in the stimulation of proliferation and differentiation of granulocyte and macrophage progenitor cells.
The neutralization dose (ND50) of this batch, as determined by the proliferation of TF-1 cells, is approximately 0.3-0.5 μg/ml in the presence of 0.5 ng/ml of recombinant human GM-CSF. The ND50 may vary depending on conditions.
Human Granulocyte colony stimulating factor (GM-CSF) is a 14.6 kDa glycoprotein, which is produced by T cells, macrophages, fibroblasts and endothelial cells. GM-CSF is involved in the stimulation of proliferation and differentiation of granulocyte and macrophage progenitor cells.
The neutralization dose (ND50) of this batch, as determined by the proliferation of TF-1 cells, is approximately 0.3-0.5 μg/ml in the presence of 0.5 ng/ml of recombinant human GM-CSF. The ND50 may vary depending on conditions.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Weak - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 1% trehalose
- Immunogen
- Recombinant protein corresponding to amino acids 14 to 144 of human GM-CSF produced in E. coli.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- P04141 Related reagents
- Entrez Gene
- CSF2 Related reagents
- GO Terms
- GO:0005125 cytokine activity
- GO:0005129 granulocyte macrophage colony-stimulating factor receptor binding
- GO:0005615 extracellular space
- GO:0006955 immune response
- GO:0008083 growth factor activity
- GO:0010744 positive regulation of macrophage derived foam cell differentiation
- GO:0032747 positive regulation of interleukin-23 production
- GO:0042523 positive regulation of tyrosine phosphorylation of Stat5 protein
- GO:0045740 positive regulation of DNA replication
- GO:0045918 negative regulation of cytolysis
- Regulatory
- For research purposes only
Applications of GM-CSF antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 0.5 | 1.0 ug/ml | |
| Functional Assays | 0.3 | 0.5 ug/ml | |
| Immunohistology - Frozen | 8.0 | 25 ug/ml | |
| Western Blotting 1 | 1.0 | 2.0 ug/ml |
- 1 4740-0955 should be used under non-reducing conditions, reducing conditions are not recommended.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
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Secondary Antibodies Available
