Find out more about A.I.den - our SearchBot

Glutamate Decarboxylase 2 antibody

ISO 9001 Quality Assurance Manufacturing | Print
Anti Glutamate Decarboxylase 2 Antibody (PrecisionAb Monoclonal Antibody) thumbnail image 1
Anti Glutamate Decarboxylase 2 Antibody (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with glutamate decarboxylase 2 antibody (VMA00153) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 134 second exposure. Arrow points to glutamate decarboxylase 2 (molecular weight 60 kDa).

Filter by Application:

WB

Mouse anti Glutamate Decarboxylase 2

Product Type
PrecisionAb Monoclonal
Isotype
IgG1
Format
Purified
Specificity
Glutamate Decarboxylase 2

Quick Links:

Antibody Quality and Performance Guarantee Antibody Quality and Performance GuaranteeAntibody Quality and
Performance Guarantee
Unrivaled technical support Unrivalled technical supportUnrivaled
Technical Suppport
Product Code Applications Pack Size List Price Your Price Qty
VMA00153
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Human glutamate decarboxylase 2 antibody recognizes glutamate decarboxylase 2, also known as 65 kDa glutamic acid decarboxylase, GAD-65, or glutamate decarboxylase 65 kDa isoform.

Encoded by the GAD2 gene, glutamate decarboxylase 2 is one of several forms of glutamic acid decarboxylase. Glutamate decarboxylase 2 is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. GAD2 may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein (provided by RefSeq, Oct 2008).

Mouse anti Human glutamate decarboxylase 2 antibody detects a band of 60 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti glutamate Decarboxylase 2 detects a band of approximately 60 kDa in Mouse Brain cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
Keyhole limpet haemocyanin (KLH) conjugated synthetic peptide sequence PGSGFWSFGSEDGSGDSEN corresponding to amino acids 4-22 within the N-terminal region of human glutamate decarboxylase 2
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
Q05329
Entrez Gene
GAD2
GO Terms
GO:0000139 Golgi membrane
GO:0005515 protein binding
GO:0042136 neurotransmitter biosynthetic process
GO:0004351 glutamate decarboxylase activity
GO:0005829 cytosol
GO:0006540 glutamate decarboxylation to succinate
GO:0007269 neurotransmitter secretion
GO:0030054 cell junction
GO:0030170 pyridoxal phosphate binding
GO:0042734 presynaptic membrane
GO:0061202 clathrin sculpted gamma-aminobutyric acid transport vesicle membrane
View more products with GLUTAMATE DECARBOXYLASE 2 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products