Glutamate Decarboxylase 2 antibody

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Anti Glutamate Decarboxylase 2 Antibody (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with glutamate decarboxylase 2 antibody (**VMA00153**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 134 second exposure. Arrow points to glutamate decarboxylase 2 (molecular weight 60 kDa).

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Mouse anti Glutamate Decarboxylase 2

Product Type: PrecisionAb Monoclonal
Isotype: IgG1
Format: Purified
Specificity: Glutamate Decarboxylase 2

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VMA00153 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti Human glutamate decarboxylase 2 antibody recognizes glutamate decarboxylase 2, also known as 65 kDa glutamic acid decarboxylase, GAD-65, or glutamate decarboxylase 65 kDa isoform.

Encoded by the GAD2 gene, glutamate decarboxylase 2 is one of several forms of glutamic acid decarboxylase. Glutamate decarboxylase 2 is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. GAD2 may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein (provided by RefSeq, Oct 2008).

Mouse anti Human glutamate decarboxylase 2 antibody detects a band of 60 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti glutamate Decarboxylase 2 detects a band of approximately 60 kDa in Mouse Brain cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody prepared by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: Keyhole limpet haemocyanin (KLH) conjugated synthetic peptide sequence PGSGFWSFGSEDGSGDSEN corresponding to amino acids 4-22 within the N-terminal region of human glutamate decarboxylase 2
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: Q05329
Entrez Gene: GAD2
GO Terms: GO:0000139 Golgi membrane; GO:0005515 protein binding; GO:0042136 neurotransmitter biosynthetic process; GO:0004351 glutamate decarboxylase activity; GO:0005829 cytosol; GO:0006540 glutamate decarboxylation to succinate; GO:0007269 neurotransmitter secretion; GO:0030054 cell junction; GO:0030170 pyridoxal phosphate binding; View More GO Terms; GO:0042734 presynaptic membrane; GO:0061202 clathrin sculpted gamma-aminobutyric acid transport vesicle membrane