Glutamate Decarboxylase 2 antibody

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Mouse anti Glutamate Decarboxylase 2

Glutamate decarboxylase 2 antibody recognizes glutamate decarboxylase 2, also known as 65 kDa glutamic acid decarboxylase or GAD-65. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Format
Purified
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00153 WB 100 µl
Mouse anti Human glutamate decarboxylase 2 antibody recognizes glutamate decarboxylase 2, also known as 65 kDa glutamic acid decarboxylase, GAD-65, or glutamate decarboxylase 65 kDa isoform.

Encoded by the GAD2 gene, glutamate decarboxylase 2 is one of several forms of glutamic acid decarboxylase. Glutamate decarboxylase 2 is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. GAD2 may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein (provided by RefSeq, Oct 2008).

Mouse anti Human glutamate decarboxylase 2 antibody detects a band of 60 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti glutamate Decarboxylase 2 detects a band of approximately 60 kDa in Mouse Brain cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Keyhole limpet haemocyanin (KLH) conjugated synthetic peptide sequence PGSGFWSFGSEDGSGDSEN corresponding to amino acids 4-22 within the N-terminal region of human glutamate decarboxylase 2
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
Q05329 Related reagents
Entrez Gene
GAD2 Related reagents
GO Terms
GO:0000139 Golgi membrane
GO:0005515 protein binding
GO:0042136 neurotransmitter biosynthetic process
GO:0004351 glutamate decarboxylase activity
GO:0005829 cytosol
GO:0006540 glutamate decarboxylation to succinate
GO:0007269 neurotransmitter secretion
GO:0030054 cell junction
GO:0030170 pyridoxal phosphate binding
GO:0042734 presynaptic membrane
GO:0061202 clathrin sculpted gamma-aminobutyric acid transport vesicle membrane
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Glutamate Decarboxylase 2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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