Glucose-6-Phosphate Dehydrogenase antibody

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Anti Glucose-6-Phosphate Dehydrogenase Antibody (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with glucose-6-phosphate dehydrogenase antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 10 second exposure. Arrow points to glucose-6-phosphate dehydrogenase (molecular weight 59 kDa)

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Mouse anti Glucose-6-Phosphate Dehydrogenase

Product Type: PrecisionAb Monoclonal
Isotype: IgG1
Format: Purified
Specificity: Glucose-6-Phosphate Dehydrogenase

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VMA00248 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti Human glucose-6-phosphate dehydrogenase antibody recognizes the glucose-6-phosphate 1-dehydrogenase, also known as glucose-6-phosphate 1-dehydrogenase.

This protein, encoded by the G6PD gene is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for G6PD (provided by RefSeq, Jul 2008).

Mouse anti Human glucose-6-phosphate 1-dehydrogenase antibody detects a band of 59 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti glucose-6-phosphate dehydrogenase detects a band of approximately 59 kDa in HeLa cell lysates.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: Purified recombinant fragment of human glucose-6-phosphate dehydrogenase expressed in E. coli
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: P11413
Entrez Gene: G6PD
GO Terms: GO:0001816 cytokine production; GO:0004345 glucose-6-phosphate dehydrogenase activity; GO:0005829 cytosol; GO:0005536 glucose binding; GO:0005813 centrosome; GO:0006749 glutathione metabolic process; GO:0006695 cholesterol biosynthetic process; GO:0009051 pentose-phosphate shunt, oxidative branch; GO:0009898 internal side of plasma membrane; View More GO Terms; GO:0010734 negative regulation of protein glutathionylation; GO:0034599 cellular response to oxidative stress; GO:0042803 protein homodimerization activity; GO:0043231 intracellular membrane-bounded organelle; GO:0043249 erythrocyte maturation; GO:0046390 ribose phosphate biosynthetic process; GO:0050661 NADP binding; GO:0051156 glucose 6-phosphate metabolic process