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Glucose-6-Phosphate Dehydrogenase antibody

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Anti Glucose-6-Phosphate Dehydrogenase Antibody (PrecisionAb Monoclonal Antibody) thumbnail image 1
Anti Glucose-6-Phosphate Dehydrogenase Antibody (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with glucose-6-phosphate dehydrogenase antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 10 second exposure. Arrow points to glucose-6-phosphate dehydrogenase (molecular weight 59 kDa)

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Mouse anti Glucose-6-Phosphate Dehydrogenase

Product Type
PrecisionAb Monoclonal
Isotype
IgG1
Format
Purified
Specificity
Glucose-6-Phosphate Dehydrogenase

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VMA00248
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Mouse anti Human glucose-6-phosphate dehydrogenase antibody recognizes the glucose-6-phosphate 1-dehydrogenase, also known as glucose-6-phosphate 1-dehydrogenase.

This protein, encoded by the G6PD gene is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for G6PD (provided by RefSeq, Jul 2008).

Mouse anti Human glucose-6-phosphate 1-dehydrogenase antibody detects a band of 59 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti glucose-6-phosphate dehydrogenase detects a band of approximately 59 kDa in HeLa cell lysates.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
Purified recombinant fragment of human glucose-6-phosphate dehydrogenase expressed in E. coli
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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Description Goat anti Mouse IgG (H/L):HRP

UniProt
P11413
Entrez Gene
G6PD
GO Terms
GO:0001816 cytokine production
GO:0004345 glucose-6-phosphate dehydrogenase activity
GO:0005829 cytosol
GO:0005536 glucose binding
GO:0005813 centrosome
GO:0006749 glutathione metabolic process
GO:0006695 cholesterol biosynthetic process
GO:0009051 pentose-phosphate shunt, oxidative branch
GO:0009898 internal side of plasma membrane
GO:0010734 negative regulation of protein glutathionylation
GO:0034599 cellular response to oxidative stress
GO:0042803 protein homodimerization activity
GO:0043231 intracellular membrane-bounded organelle
GO:0043249 erythrocyte maturation
GO:0046390 ribose phosphate biosynthetic process
GO:0050661 NADP binding
GO:0051156 glucose 6-phosphate metabolic process
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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