Glucose-6-Phosphate Dehydrogenase antibody

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Mouse anti Glucose-6-Phosphate Dehydrogenase

Glucose-6-phosphate dehydrogenase antibody recognizes glucose-6-phosphate 1-dehydrogenase. This antibody has been extensively validated for western blotting using whole cell lysates.

Glucose-6-Phosphate Dehydrogenase Antibody with Control Lysate

Glucose-6-phosphate dehydrogenase antibody recognizes glucose-6-phosphate 1-dehydrogenase. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00248 WB 100 µl
VMA00248KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human glucose-6-phosphate dehydrogenase antibody recognizes the glucose-6-phosphate 1-dehydrogenase, also known as glucose-6-phosphate 1-dehydrogenase.

This protein, encoded by the G6PD gene is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for G6PD (provided by RefSeq, Jul 2008).

Mouse anti Human glucose-6-phosphate 1-dehydrogenase antibody detects a band of 59 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti glucose-6-phosphate dehydrogenase detects a band of approximately 59 kDa in HeLa cell lysates
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
Preparation
20μl Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Purified recombinant fragment of human glucose-6-phosphate dehydrogenase expressed in E. coli
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HeLa lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P11413 Related reagents
Entrez Gene
G6PD Related reagents
GO Terms
GO:0001816 cytokine production
GO:0004345 glucose-6-phosphate dehydrogenase activity
GO:0005829 cytosol
GO:0005536 glucose binding
GO:0005813 centrosome
GO:0006749 glutathione metabolic process
GO:0006695 cholesterol biosynthetic process
GO:0009051 pentose-phosphate shunt, oxidative branch
GO:0009898 internal side of plasma membrane
GO:0010734 negative regulation of protein glutathionylation
GO:0034599 cellular response to oxidative stress
GO:0042803 protein homodimerization activity
GO:0043231 intracellular membrane-bounded organelle
GO:0043249 erythrocyte maturation
GO:0046390 ribose phosphate biosynthetic process
GO:0050661 NADP binding
GO:0051156 glucose 6-phosphate metabolic process
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Glucose-6-Phosphate Dehydrogenase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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