Glucose-6-Phosphate Dehydrogenase antibody
Mouse anti Glucose-6-Phosphate Dehydrogenase
Glucose-6-phosphate dehydrogenase antibody recognizes glucose-6-phosphate 1-dehydrogenase. This antibody has been extensively validated for western blotting using whole cell lysates.
Glucose-6-Phosphate Dehydrogenase Antibody with Control Lysate
Glucose-6-phosphate dehydrogenase antibody recognizes glucose-6-phosphate 1-dehydrogenase. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00248 | WB | 100 µl | ||||
| VMA00248KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human glucose-6-phosphate dehydrogenase antibody recognizes the glucose-6-phosphate 1-dehydrogenase, also known as glucose-6-phosphate 1-dehydrogenase.
This protein, encoded by the G6PD gene is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for G6PD (provided by RefSeq, Jul 2008).
Mouse anti Human glucose-6-phosphate 1-dehydrogenase antibody detects a band of 59 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
This protein, encoded by the G6PD gene is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for G6PD (provided by RefSeq, Jul 2008).
Mouse anti Human glucose-6-phosphate 1-dehydrogenase antibody detects a band of 59 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti glucose-6-phosphate dehydrogenase detects a band of approximately 59 kDa in HeLa cell lysates
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
- Preparation
- 20μl Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Purified recombinant fragment of human glucose-6-phosphate dehydrogenase expressed in E. coli
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HeLa lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P11413 Related reagents
- Entrez Gene
- G6PD Related reagents
- GO Terms
- GO:0001816 cytokine production
- GO:0004345 glucose-6-phosphate dehydrogenase activity
- GO:0005829 cytosol
- GO:0005536 glucose binding
- GO:0005813 centrosome
- GO:0006749 glutathione metabolic process
- GO:0006695 cholesterol biosynthetic process
- GO:0009051 pentose-phosphate shunt, oxidative branch
- GO:0009898 internal side of plasma membrane
- GO:0010734 negative regulation of protein glutathionylation
- GO:0034599 cellular response to oxidative stress
- GO:0042803 protein homodimerization activity
- GO:0043231 intracellular membrane-bounded organelle
- GO:0043249 erythrocyte maturation
- GO:0046390 ribose phosphate biosynthetic process
- GO:0050661 NADP binding
- GO:0051156 glucose 6-phosphate metabolic process
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Glucose-6-Phosphate Dehydrogenase antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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