Glucagon antibody

Mouse anti Glucagon

Product Type
PrecisionAb Monoclonal
Isotype
IgG1
Format
Purified
Specificity
Glucagon

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VMA00467
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl loader
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Mouse anti Human glucagon antibody recognizes the hormone glucagon.The protein encoded by GCG gene is actually a preproprotein that is cleaved into four distinct mature peptides. One of these, glucagon, is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. Glucagon is a ligand for a specific G-protein linked receptor whose signalling pathway controls cell proliferation. Two of the other peptides are secreted from gut endocrine cells and promote nutrient absorption through distinct mechanisms. Finally, the fourth peptide is similar to glicentin, an active enteroglucagon. (provided by RefSeq, Jul 2008).

Mouse anti Human glucagon antibody detects a band of 21 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti glucagon detects a band of approximately 21 kDa in HepG2 cell lysate.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
KLH conjugated synthetic peptide between amino acids 119-148 of human glucagon
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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Description Goat anti Mouse IgG (H/L):HRP

UniProt
P01275
Entrez Gene
GCG
GO Terms
GO:0005886 plasma membrane
GO:0005576 extracellular region
GO:0005179 hormone activity
GO:0005625 soluble fraction
GO:0006112 energy reserve metabolic process
GO:0007186 G-protein coupled receptor protein signaling pathway
GO:0007631 feeding behavior
GO:0050796 regulation of insulin secretion
GO:0008283 cell proliferation
GO:0071377 cellular response to glucagon stimulus
View more products with GLUCAGON specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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