GFAP antibody

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Mouse anti GFAP

Mouse anti-human GFAP antibody recognizes glial fibrillary acidic protein. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Format
Purified
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00478 WB 100 µl
Mouse anti Human GFAP antibody recognizes GFAP also known as glial fibrillary acidic protein.

The GFAP gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in GFAP cause Alexander disease (AXLDRD), a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms (provided by RefSeq, Oct 2008).

Mouse anti Human GFAP antibody detects a band of 50 kDa. The antibody has been extensively validated for western blotting using tissue and whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti GFAP detects a band of approximately 50 kDa in mouse brain tissue lysate
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to amino acids 411-422 (KTVEMRDGEVIK) of human GFAP
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P14136 Related reagents
Entrez Gene
GFAP Related reagents
Acknowledgements
PrecisionAbâ„¢ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of GFAP antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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