GFAP antibody | GF-05
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Mouse anti Human Glial Fibrillary Acidic Protein antibody, clone GF-05 recognizes humanm glial fibrillary acidic protein (GFAP). GFAP is a 432 amino acid intermediate filament protein. Mouse anti Human Glial Fibrillary Acidic Protein antibody, clone GF-05 recognisez ~38-45 kDa neurofilaments in immunoblots. Mouse anti Human Glial Fibrillary Acidic Protein antibody, clone GF-05 is also reactive in ELISA with antigen coating at 5 μg/ml and may react with GFAP from other species.
Mutations in the GFAP gene may result in presentation of Alexander disease (ALXDRD), a rare neurological condition affecting infants and characterized by myelination failure leading to early death (Brenner et al. 2001)
Mutations in the GFAP gene may result in presentation of Alexander disease (ALXDRD), a rare neurological condition affecting infants and characterized by myelination failure leading to early death (Brenner et al. 2001)
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Cynomolgus monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Native GFAP purified from human brain.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
Applications of GFAP antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Western Blotting |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for GFAP antibody
-
Burbaeva, G.Sh. et al. (2007) Systemic neurochemical alterations in schizophrenic brain: glutamate metabolism in focus.
Neurochem Res. 32: 1434-44. -
Gil-Perotin, S. et al. (2009) Ultrastructure of the subventricular zone in Macaca fascicularis and evidence of a mouse-like migratory stream.
J Comp Neurol. 514: 533-54. -
Wei, P. et al. (2013) Serum GFAP autoantibody as an ELISA-detectable glioma marker.
Tumour Biol. 34 (4): 2283-92. -
Garbayo, E. et al. (2016) Brain delivery of microencapsulated GDNF induces functional and structural recovery in parkinsonian monkeys.
Biomaterials. 110: 11-23. -
Martinez-Valbuena, M. et al. (2019) Amylin as a potential link between type 2 diabetes and alzheimer disease.
Ann Neurol. 86 (4): 539-51. -
Carmona-Abellan, M. et al. (2021) Microglia is associated with p-Tau aggregates in the olfactory bulb of patients with neurodegenerative diseases.
Neurol Sci. 42 (4): 1473-82.
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