GFAP antibody | GF-05

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Mouse anti Human GFAP

Product Type
Monoclonal Antibody
Clone
GF-05
Isotype
IgG2b
Product Code Applications Pack Size List Price Quantity
4650-0309
Datasheet
E WB
MSDS
0.2 mg loader

Mouse anti Human Glial Fibrillary Acidic Protein antibody, clone GF-05 recognizes humanm glial fibrillary acidic protein (GFAP). GFAP is a 432 amino acid intermediate filament protein. Mouse anti Human Glial Fibrillary Acidic Protein antibody, clone GF-05 recognisez ~38-45 kDa neurofilaments in immunoblots. Mouse anti Human Glial Fibrillary Acidic Protein antibody, clone GF-05 is also reactive in ELISA with antigen coating at 5 μg/ml and may react with GFAP from other species.

Mutations in the GFAP gene may result in presentation of Alexander disease (ALXDRD), a rare neurological condition affecting infants and characterized by myelination failure leading to early death (Brenner et al. 2001)

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Cynomolgus monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Native GFAP purified from human brain.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.

More Information

UniProt
P14136 Related reagents
Entrez Gene
GFAP Related reagents
Regulatory
For research purposes only

Applications of GFAP antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Human anti Mouse IgG2b:FITC HCA038F F 0.1 mg loader
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Product Specific References

References for GFAP antibody

  1. Burbaeva, G.Sh. et al. (2007) Systemic neurochemical alterations in schizophrenic brain: glutamate metabolism in focus.
    Neurochem Res. 32: 1434-44.
  2. Gil-Perotin, S. et al. (2009) Ultrastructure of the subventricular zone in Macaca fascicularis and evidence of a mouse-like migratory stream.
    J Comp Neurol. 514: 533-54.
  3. Wei, P. et al. (2013) Serum GFAP autoantibody as an ELISA-detectable glioma marker.
    Tumour Biol. 34 (4): 2283-92.
  4. Garbayo, E. et al. (2016) Brain delivery of microencapsulated GDNF induces functional and structural recovery in parkinsonian monkeys.
    Biomaterials. 110: 11-23.
  5. Martinez-Valbuena, M. et al. (2019) Amylin as a potential link between type 2 diabetes and alzheimer disease.
    Ann Neurol. 86 (4): 539-51.