GATA3 antibody | OTI8H4
Mouse anti GATA3
Mouse anti human GATA3 antibody recognizes the trans-acting T-cell-specific transcription factor GATA-3, also known as GATA-binding factor 3. This antibody has been extensively validated for western blotting using whole cell lysates.
GATA3 Antibody with Control Lysate
Mouse anti human GATA3 antibody recognizes the trans-acting T-cell-specific transcription factor GATA-3, also known as GATA-binding factor 3. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- OTI8H4
- Isotype
- IgG1
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00377 | WB | 100 µl | ||||
| VMA00377KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human GATA3 antibody recognizes the transcription factor GATA-3, also known as GATA-binding factor 3 and trans-acting T-cell-specific transcription factor GATA-3.
The GATA3 gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains two GATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelial cell biology. Defects in GATA3 are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia (provided by RefSeq, Nov 2009).
Mouse anti Human GATA3 antibody detects a band of 48 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The GATA3 gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains two GATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelial cell biology. Defects in GATA3 are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia (provided by RefSeq, Nov 2009).
Mouse anti Human GATA3 antibody detects a band of 48 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography from tissue culture supernatant
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- Recombinant protein fragment corresponding to aa 155-443 of human GATA3 (NP_002042) produced in E.coli
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- P23771 Related reagents
- Entrez Gene
- GATA3 Related reagents
- GO Terms
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0006952 defense response
- GO:0007596 blood coagulation
- GO:0005654 nucleoplasm
- GO:0005730 nucleolus
- GO:0006355 regulation of transcription, DNA-dependent
- GO:0006366 transcription from RNA polymerase II promoter
- GO:0009653 anatomical structure morphogenesis
- GO:0008134 transcription factor binding
- GO:0008270 zinc ion binding
- GO:0043565 sequence-specific DNA binding
- GO:0043627 response to estrogen stimulus
- GO:0071837 HMG box domain binding
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of GATA3 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
- Western Blotting
- Anti GATA3 detects a band of approximately 48 kDa in Jurkat cell lysates
- Western Blotting
- Anti GATA3 detects a band of approximately 48 kDa in Jurkat cell lysates
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
