GATA3 antibody | OTI8H4

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Anti GATA3 Antibody, clone OTI8H4 (PrecisionAb™ Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with GATA3 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 25 second exposure. Arrow points to GATA3 (molecular weight 48 kDa)

Mouse anti GATA3

Mouse anti human GATA3 antibody recognizes the trans-acting T-cell-specific transcription factor GATA-3, also known as GATA-binding factor 3. This antibody has been extensively validated for western blotting using whole cell lysates.

GATA3 Antibody with Control Lysate

Product Type: PrecisionAb™ Monoclonal
Clone: OTI8H4
Isotype: IgG1
Format: Purified

Product Code	Applications	Pack Size
VMA00377	WB	100 µl
VMA00377KT	WB	2 Westerns
With Lysate		400µg

Summary
Secondary Antibodies

Mouse anti Human GATA3 antibody recognizes the transcription factor GATA-3, also known as GATA-binding factor 3 and trans-acting T-cell-specific transcription factor GATA-3.

The GATA3 gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains two GATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelial cell biology. Defects in GATA3 are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia (provided by RefSeq, Nov 2009).

Mouse anti Human GATA3 antibody detects a band of 48 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species: Human
Western Blotting: Anti GATA3 detects a band of approximately 48 kDa in Jurkat cell lysates
Product Form: Purified IgG - liquid
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography from tissue culture supernatant
Preparation: 20μl Mouse monoclonal antibody purified by affinity chromatography from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Immunogen: Recombinant protein fragment corresponding to aa 155-443 of human GATA3 (NP_002042) produced in E.coli
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition: 400μg Jurkat lysate lyophilized in RIPA buffer
Lysate Reconstitution: - If using DDT reconstitute the lyophilized lysate with 190μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Storage: Antibody: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life: As supplied, 12 months from date of despatch.
Shelf Life: As supplied, 12 months from date of despatch

More Information

UniProt: P23771 Related reagents
Entrez Gene: GATA3 Related reagents
GO Terms: GO:0003700 sequence-specific DNA binding transcription factor activity; GO:0006952 defense response; GO:0007596 blood coagulation; GO:0005654 nucleoplasm; GO:0005730 nucleolus; GO:0006355 regulation of transcription, DNA-dependent; GO:0006366 transcription from RNA polymerase II promoter; GO:0009653 anatomical structure morphogenesis; GO:0008134 transcription factor binding; GO:0008270 zinc ion binding; GO:0043565 sequence-specific DNA binding; GO:0043627 response to estrogen stimulus; GO:0071837 HMG box domain binding
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory: For research purposes only

Applications of GATA3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB

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