GARS antibody | OTI4H8

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Anti GARS Antibody, clone OTI4H8 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti GARS Antibody, clone OTI4H8 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti GARS Antibody, clone OTI4H8 (PrecisionAb Monoclonal Antibody) thumbnail image 3

Anti GARS Antibody, clone OTI4H8 (PrecisionAb Monoclonal Antibody) thumbnail image 4

Anti GARS Antibody, clone OTI4H8 (PrecisionAb Monoclonal Antibody) thumbnail image 5

Anti GARS Antibody, clone OTI4H8 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with Mouse anti Human GARS antibody, clone OTI4H8 (**VMA00198**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 7 second exposure. Arrow points to GARS (molecular weight 83 kDa).

Anti GARS Antibody, clone OTI4H8 (PrecisionAb Monoclonal Antibody) gallery image 2 — Western blot analysis of GARS knockout 293T and wild type 293T (WT) whole cell lysates probed with Mouse anti GARS antibody (**VMA00198**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and visualized on the ChemiDoc MP with a 3 second exposure. Tubulin was detected with hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a 30 second exposure.

Anti GARS Antibody, clone OTI4H8 (PrecisionAb Monoclonal Antibody) gallery image 3 — Normalized signal intensity of GARS from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.

Anti GARS Antibody, clone OTI4H8 (PrecisionAb Monoclonal Antibody) gallery image 4 — Western blot analysis of whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against GARS (1 & 2) or a scrambled siRNA (-) probed with Mouse anti GARS antibody (**VMA00198**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, 1705047) and hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a 67 second (chemiluminescent channel) and 30 second exposure

Anti GARS Antibody, clone OTI4H8 (PrecisionAb Monoclonal Antibody) gallery image 5 — Normalized signal intensity of GARS from whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against GARS (1 & 2) or a scrambled siRNA (-). Signal intensity was normalized to the tubulin signal.

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Mouse anti GARS

Product Type: PrecisionAb Monoclonal
Clone: OTI4H8
Isotype: IgG2b
Format: Purified
Specificity: GARS

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VMA00198 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti Human GARS antibody, clone OTI4H8 recognizes GARS, also known as AP-4-A synthetase, Charcot-Marie-Tooth neuropathy 2D, Charcot-Marie-Tooth neuropathy, neuronal type, D, diadenosine tetraphosphate synthetase, glycine tRNA ligase and glycine--tRNA ligase.

The GARS gene encodes one of the aminoacyl-tRNA synthetases that charge tRNAs with their cognate amino acids. The encoded enzyme is an (alpha)2 dimer which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis (provided by RefSeq, Jul 2008).

Mouse anti Human GARS antibody, clone OTI4H8 detects a band of 83 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti GARS detects a band of approximately 83 kDa in K562 cell lysates.
Product Form: Purified IgG - liquid.
Preparation: Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Immunogen: Full length recombinant human GARS (NP_002038) produced in HEK293T cells
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: P41250
Entrez Gene: GARS
GO Terms: GO:0005829 cytosol; GO:0004820 glycine-tRNA ligase activity; GO:0005524 ATP binding; GO:0005625 soluble fraction; GO:0005759 mitochondrial matrix; GO:0006426 glycyl-tRNA aminoacylation; GO:0008219 cell death; GO:0015966 diadenosine tetraphosphate biosynthetic process; GO:0046983 protein dimerization activity; View More GO Terms