GADD45A antibody | GH02/2F4

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Mouse anti GADD45A

Product Type
PrecisionAb Monoclonal
Clone
GH02/2F4
Isotype
IgG2b
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00965
Datasheet
IP WB
SDS
100 µl loader

Mouse anti GADD45A antibody recognizes growth arrest and DNA damage-inducible protein GADD45 alpha, also known as DDIT-1, GADD45. GADD45A is a stress response protein belonging to the DNA damage-inducible 45 family and promotes active DNA methylation (Arab et al. 2019). In response to physiological or environmental stress GADD45A assists in DNA repair, cell cycle arrest and apoptosis, and other growth regulatory processes (Liu et al. 2018). A broad range of signaling pathways are linked to GADD45S, including p38 and JNK MAP kinases, BRCA1, FOXOA3 and ATF4. Accordingly, GADD45A has been shown to interact with a variety of proteins including cdc2. GADD45A is a direct transcriptional target of the tumor suppressor FOXO3A (Han et al. 2019). It is also a downstream target of p53 and BRCA1. Because it plays a role in genome stability, GADD45A has been implicated in a range of cancer types. Elevated GADD45A levels have been observed in esophageal squamous cell carcinoma and breast cancer, for example (Wang et al. 2018).

Product Details

Target Species
Human
Western Blotting
Mouse anti GADD45A detects a band of approximately 23 kDa in Jurkat cell lysates
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-165 of human GADD45A
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P24522
Entrez Gene
GADD45A
GO Terms
GO:0000079 regulation of cyclin-dependent protein kinase activity
GO:0005515 protein binding
GO:0006915 apoptosis
GO:0005634 nucleus
GO:0006281 DNA repair
GO:0007050 cell cycle arrest
GO:0010670 positive regulation of oxygen and reactive oxygen species metabolic process
GO:0042770 signal transduction in response to DNA damage
GO:0071479 cellular response to ionizing radiation
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of GADD45A antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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