FYN antibody
Mouse anti Human FYN antibody recognizes FYN, a member of the src family of non receptor protein tyrosine kinases, expressed by T-cells and natural killer (NK) cells, and highly expressed in brain.
FYN plays a vital role in thymocyte development and T cell activation and is essential for TCR-mediated signal transduction through association with the T-cell receptor (TCR) eta chain/CD3 complex. This association, results in protein kinase activation and the subsequent protein tyrosine phosphorylation of multiple immunoreceptor tyrosine-based activation motifs (ITAMs), which triggers the phosphorylation of further substrates downstream in the T-cell signaling cascade.
Recent studies into Alzheimer’s disease have implicated FYN in neurodegeneration, through the tyrosine phosphorylation of the microtubule-associated protein tau, a prominent component of neurofibrillary tangles.
Mouse anti Human FYN antibody recognizes FYN as a band of ~61 kDa in western blotting in multiple cell line lysates including myeloid, lymphoid, neuronal, adenocarcinoma, sarcoma and embryonic kidney.
FYN plays a vital role in thymocyte development and T cell activation and is essential for TCR-mediated signal transduction through association with the T-cell receptor (TCR) eta chain/CD3 complex. This association, results in protein kinase activation and the subsequent protein tyrosine phosphorylation of multiple immunoreceptor tyrosine-based activation motifs (ITAMs), which triggers the phosphorylation of further substrates downstream in the T-cell signaling cascade.
Recent studies into Alzheimer’s disease have implicated FYN in neurodegeneration, through the tyrosine phosphorylation of the microtubule-associated protein tau, a prominent component of neurofibrillary tangles.
Mouse anti Human FYN antibody recognizes FYN as a band of ~61 kDa in western blotting in multiple cell line lysates including myeloid, lymphoid, neuronal, adenocarcinoma, sarcoma and embryonic kidney.
Product Details
- Target Species
- Human
- Western Blotting
- Anti FYN antibody detects a band of approximately 61 kDa in HEK293 cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Bacterially expressed recombinant fragment of human FYN aa 7-176
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P06241
- Entrez Gene
- FYN
- GO Terms
- GO:0005886 plasma membrane
- GO:0001948 glycoprotein binding
- GO:0005829 cytosol
- GO:0004715 non-membrane spanning protein tyrosine kinase activity
- GO:0046872 metal ion binding
- GO:0005524 ATP binding
- GO:0005768 endosome
- GO:0006468 protein phosphorylation
- GO:0006816 calcium ion transport
- GO:0007243 intracellular protein kinase cascade
- GO:0007411 axon guidance
- GO:0007612 learning
- GO:0007631 feeding behavior
- GO:0042802 identical protein binding
- GO:0016032 viral reproduction
- GO:0030168 platelet activation
- GO:0031295 T cell costimulation
- GO:0044419 interspecies interaction between organisms
- GO:0050690 regulation of defense response to virus by virus
- GO:0050852 T cell receptor signaling pathway
- GO:0050900 leukocyte migration
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of FYN antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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