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FYN antibody

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Anti FYN Antibody (PrecisionAb Monoclonal Antibody) thumbnail image 1
Anti FYN Antibody (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with Mouse anti Human FYN antibody (VMA00053)followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 197 second exposure. Arrow points to FYN (molecular weight 61 kDa).

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WB

Mouse anti FYN

Product Type
PrecisionAb Monoclonal
Isotype
IgG2b
Format
Purified
Specificity
FYN

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Product Code Applications Pack Size List Price Your Price Qty
VMA00053
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SDS Safety Datasheet SDS
WB 100 µl loader
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Mouse anti Human FYN antibody recognizes FYN, a member of the src family of non receptor protein tyrosine kinases, expressed by T-cells and natural killer (NK) cells, and highly expressed in brain.

FYN plays a vital role in thymocyte development and T cell activation and is essential for TCR-mediated signal transduction through association with the T-cell receptor (TCR) eta chain/CD3 complex. This association, results in protein kinase activation and the subsequent protein tyrosine phosphorylation of multiple immunoreceptor tyrosine-based activation motifs (ITAMs), which triggers the phosphorylation of further substrates downstream in the T-cell signaling cascade.

FYN is involved in neurodegeneration, through the tyrosine phosphorylation of the microtubule-associated protein tau, a prominent component of neurofibrillary tangles.

Mouse anti Human FYN antibody recognizes FYN as a band of ~61 kDa in western blotting in multiple cell line lysates including myeloid, lymphoid, neuronal, adenocarcinoma, sarcoma and embryonic kidney.		 PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti FYN antibody detects a band of approximately 61 kDa in HEK293 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
Bacterially expressed recombinant fragment of human FYN aa 7-176
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
P06241
Entrez Gene
FYN
GO Terms
GO:0005886 plasma membrane
GO:0001948 glycoprotein binding
GO:0005829 cytosol
GO:0004715 non-membrane spanning protein tyrosine kinase activity
GO:0046872 metal ion binding
GO:0005524 ATP binding
GO:0005768 endosome
GO:0006468 protein phosphorylation
GO:0006816 calcium ion transport
GO:0007243 intracellular protein kinase cascade
GO:0007411 axon guidance
GO:0007612 learning
GO:0007631 feeding behavior
GO:0042802 identical protein binding
GO:0016032 viral reproduction
GO:0030168 platelet activation
GO:0031295 T cell costimulation
GO:0044419 interspecies interaction between organisms
GO:0050690 regulation of defense response to virus by virus
GO:0050852 T cell receptor signaling pathway
GO:0050900 leukocyte migration
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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