FOXP1 antibody | JC12

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Mouse anti FOXP1

Product Type
Monoclonal Antibody
Clone
JC12
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2485T C P* WB 25 µg
MCA2485GA C P* WB 0.1 mg
MCA2485 C P* WB 0.2 mg
Mouse anti FOXP1 antibody, clone JC12 recognizes human FOXP1, also known as Forkhead box protein P1, Mac-1-regulated forkhead or MFH. FOXP1 is a 677 amino acid~85 kDa transcriptional repressor and member of the winged helix/forkhead transcription factor family. The FOXP1 transcription factor is widely expressed in human tissues and is predominantly localised to the nucleus. However, the level of expression does vary between cells and between different tissues. The expression of FOXP1 is reported to be abnormal in a range of solid tumours (Giatromanolaki et al. 2006).

Mouse anti FOXP1 antibody, clone JC12 does not recognize closely related molecules FOXP2, FOXP3 or FOXP4.

Mouse anti FOXP1 antibody, clone JC12 was derived from a fusion intended to isolate antibodies against human Bcl-X. However the JC12 clone did not bind to the immunogen and was considered to be from a B cell secreting an autoantibody expanded during the fusion process. Sequencing of the bound target indicated that the clone JC12 recognizes both human and murine FOXP1 (Banham et al. 2001).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
A synthetic peptide corresponding to a region adjacent to the C-terminus of human Bcl-2-like protein 1 (Bcl-X) with the sequence NAAAESRKGQERFN-C (c-terminal cysteine for coupling). Note: This immunizing sequence is not pertinent to the isolation of the derived clone (see specificity).
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q9H334 Related reagents
Entrez Gene
FOXP1 Related reagents
GO Terms
GO:0002053 positive regulation of mesenchymal cell proliferation
GO:0002329 pre-B cell differentiation
GO:0002639 positive regulation of immunoglobulin production
GO:0003682 chromatin binding
GO:0003690 double-stranded DNA binding
GO:0003704 specific RNA polymerase II transcription factor activity
GO:0003705 sequence-specific enhancer binding RNA polymerase II transcription factor activity
GO:0005737 cytoplasm
GO:0005667 transcription factor complex
GO:0009790 embryo development
GO:0007389 pattern specification process
GO:0007519 skeletal muscle tissue development
GO:0008134 transcription factor binding
GO:0008270 zinc ion binding
GO:0008301 DNA bending activity
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010843 promoter binding
GO:0016563 transcription activator activity
GO:0016566 specific transcriptional repressor activity
GO:0033152 immunoglobulin V(D)J recombination
GO:0042803 protein homodimerization activity
GO:0046982 protein heterodimerization activity
GO:0048745 smooth muscle tissue development
GO:0051090 regulation of transcription factor activity
GO:0055007 cardiac muscle cell differentiation
GO:0060501 positive regulation of epithelial cell proliferation involved in lung morphogenesis
Regulatory
For research purposes only

Applications of FOXP1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen
Immunohistology - Paraffin 1 1/200 1/500
Western Blotting
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
    1mM EDTA pH8.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human tonsil.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F 0.1 mg F
Human anti Mouse IgG2a:HRP HCA037P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Histar Detection System STAR3000A 50 Tests C P
Histar Detection System STAR3000B 150 Tests C P
Histar Detection System STAR3000C 500 Tests C P

Application Based External Images

Immunohistology - Paraffin

Product Specific References

References for FOXP1 antibody

  1. Banham, A.H. et al. (2005) Expression of the FOXP1 transcription factor is strongly associated with inferior survival in patients with diffuse large B-cell lymphoma.
    Clin Cancer Res. 11 (3): 1065-72.
  2. Banham, A.H. et al. (2001) The FOXP1 winged helix transcription factor is a novel candidate tumor suppressor gene on chromosome 3p.
    Cancer Res. 61 (24): 8820-9.
  3. Yamada, S. et al. (2012) Forkhead box P1 overexpression and its clinicopathologic significance in peripheral T-cell lymphoma, not otherwise specified.
    Hum Pathol. 43 (8): 1322-7.
  4. Yu, B. et al. (2011) FOXP1 expression and its clinicopathologic significance in nodal and extranodal diffuse large B-cell lymphoma.
    Ann Hematol. 90 (6): 701-8.
  5. Hu, C.R. et al. (2013) Both FOXP1 and p65 expression are adverse risk factors in diffuse large B-cell lymphoma: a retrospective study in China.
    Acta Histochem. 115 (2): 137-43.
  6. Hoefnagel, J.J. et al. (2006) Expression of B-cell transcription factors in primary cutaneous B-cell lymphoma.
    Mod Pathol. 19 (9): 1270-6.
  7. Marafioti, T. et al. (2008) Novel markers of normal and neoplastic human plasmacytoid dendritic cells.
    Blood. 111 (7): 3778-92.
  8. Yu, B.H. et al. (2018) Cytoplasmic FOXP1 expression is correlated with ER and calpain II expression and predicts a poor outcome in breast cancer.
    Diagn Pathol. 13 (1): 36.