FOXA2 antibody | OTI3C10
Mouse anti FOXA2
FOXA2 antibody recognizes hepatocyte nuclear factor 3-beta, also known as HNF-3-beta, HNF-3B, TCF-3B, forkhead box protein A2, hepatic nuclear factor-3-beta or transcription factor 3B. This antibody has been extensively validated for western blotting using whole cell lysates.
FOXA2 Antibody with Control Lysate
FOXA2 antibody recognizes hepatocyte nuclear factor 3-beta, also known as HNF-3-beta, HNF-3B, TCF-3B, forkhead box protein A2, hepatic nuclear factor-3-beta or transcription factor 3B. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- OTI3C10
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00137 | WB | 100 µl | ||||
| VMA00137KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human FOXA2 antibody recognizes FOXA2, also known as HNF-3-beta, HNF-3B, TCF-3B, forkhead box protein A2, hepatic nuclear factor-3-beta, hepatocyte nuclear factor 3, beta, hepatocyte nuclear factor 3-beta and transcription factor 3B.
The FOXA2 gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific genes such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. FOXA2 has been linked to sporadic cases of maturity-onset diabetes of the young. Transcript variants encoding different isoforms have been identified for FOXA2 (provided by RefSeq, Oct 2008).
Mouse anti Human FOXA2 antibody detects a band of 50 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The FOXA2 gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific genes such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. FOXA2 has been linked to sporadic cases of maturity-onset diabetes of the young. Transcript variants encoding different isoforms have been identified for FOXA2 (provided by RefSeq, Oct 2008).
Mouse anti Human FOXA2 antibody detects a band of 50 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti FOXA2 detects a band of approximately 50 kDa in HepG2 cell lysates.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography from ascites.
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatography from ascites.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- Recombinant protein expressed in E.coli corresponding to amino acids 300-458 of human FOXA2
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HepG2 lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- Q9Y261 Related reagents
- Entrez Gene
- FOXA2 Related reagents
- GO Terms
- GO:0001701 in utero embryonic development
- GO:0001705 ectoderm formation
- GO:0003690 double-stranded DNA binding
- GO:0003704 specific RNA polymerase II transcription factor activity
- GO:0003705 sequence-specific enhancer binding RNA polymerase II transcription factor activity
- GO:0005737 cytoplasm
- GO:0005667 transcription factor complex
- GO:0007492 endoderm development
- GO:0008134 transcription factor binding
- GO:0008301 DNA bending activity
- GO:0009952 anterior/posterior pattern formation
- GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
- GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
- GO:0010843 promoter binding
- GO:0016563 transcription activator activity
- GO:0016566 specific transcriptional repressor activity
- GO:0016568 chromatin modification
- GO:0019904 protein domain specific binding
- GO:0021533 cell differentiation in hindbrain
- GO:0021779 oligodendrocyte cell fate commitment
- GO:0021904 dorsal/ventral neural tube patterning
- GO:0030193 regulation of blood coagulation
- GO:0031018 endocrine pancreas development
- GO:0032289 central nervous system myelin formation
- GO:0045665 negative regulation of neuron differentiation
- GO:0045666 positive regulation of neuron differentiation
- GO:0048665 neuron fate specification
- GO:0051090 regulation of transcription factor activity
- GO:0060441 epithelial tube branching involved in lung morphogenesis
- GO:0060487 lung epithelial cell differentiation
- GO:0070741 response to interleukin-6
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of FOXA2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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