FOXA2 antibody | OTI3C10

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Mouse anti FOXA2

FOXA2 antibody recognizes hepatocyte nuclear factor 3-beta, also known as HNF-3-beta, HNF-3B, TCF-3B, forkhead box protein A2, hepatic nuclear factor-3-beta or transcription factor 3B. This antibody has been extensively validated for western blotting using whole cell lysates.

FOXA2 Antibody with Control Lysate

FOXA2 antibody recognizes hepatocyte nuclear factor 3-beta, also known as HNF-3-beta, HNF-3B, TCF-3B, forkhead box protein A2, hepatic nuclear factor-3-beta or transcription factor 3B. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI3C10
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00137 WB 100 µl
VMA00137KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human FOXA2 antibody recognizes FOXA2, also known as HNF-3-beta, HNF-3B, TCF-3B, forkhead box protein A2, hepatic nuclear factor-3-beta, hepatocyte nuclear factor 3, beta, hepatocyte nuclear factor 3-beta and transcription factor 3B.

The FOXA2 gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific genes such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. FOXA2 has been linked to sporadic cases of maturity-onset diabetes of the young. Transcript variants encoding different isoforms have been identified for FOXA2 (provided by RefSeq, Oct 2008).

Mouse anti Human FOXA2 antibody detects a band of 50 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Recombinant protein expressed in E.coli corresponding to amino acids 300-458 of human FOXA2

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
Q9Y261 Related reagents
Entrez Gene
FOXA2 Related reagents
GO Terms
GO:0001701 in utero embryonic development
GO:0001705 ectoderm formation
GO:0003690 double-stranded DNA binding
GO:0003704 specific RNA polymerase II transcription factor activity
GO:0003705 sequence-specific enhancer binding RNA polymerase II transcription factor activity
GO:0005737 cytoplasm
GO:0005667 transcription factor complex
GO:0007492 endoderm development
GO:0008134 transcription factor binding
GO:0008301 DNA bending activity
GO:0009952 anterior/posterior pattern formation
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010843 promoter binding
GO:0016563 transcription activator activity
GO:0016566 specific transcriptional repressor activity
GO:0016568 chromatin modification
GO:0019904 protein domain specific binding
GO:0021533 cell differentiation in hindbrain
GO:0021779 oligodendrocyte cell fate commitment
GO:0021904 dorsal/ventral neural tube patterning
GO:0030193 regulation of blood coagulation
GO:0031018 endocrine pancreas development
GO:0032289 central nervous system myelin formation
GO:0045665 negative regulation of neuron differentiation
GO:0045666 positive regulation of neuron differentiation
GO:0048665 neuron fate specification
GO:0051090 regulation of transcription factor activity
GO:0060441 epithelial tube branching involved in lung morphogenesis
GO:0060487 lung epithelial cell differentiation
GO:0070741 response to interleukin-6
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of FOXA2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti FOXA2 detects a band of approximately 50 kDa in HepG2 cell lysates.
Western Blotting
Anti FOXA2 detects a band of approximately 50 kDa in HepG2 cell lysates.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB