FOXA2 antibody | OTI3C10

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Anti FOXA2 Antibody, clone OTI3C10 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with Mouse anti Human FOXA2 antibody (**VMA00137**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 46 second exposure. Arrow points to FOXA2 (molecular weight 50 kDa).

Mouse anti FOXA2

Product Type: PrecisionAb Monoclonal
Clone: OTI3C10
Isotype: IgG1
Format: Purified

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
VMA00137 Datasheet	WB SDS	100 µl	Log in		Get Quote

Mouse anti Human FOXA2 antibody recognizes FOXA2, also known as HNF-3-beta, HNF-3B, TCF-3B, forkhead box protein A2, hepatic nuclear factor-3-beta, hepatocyte nuclear factor 3, beta, hepatocyte nuclear factor 3-beta and transcription factor 3B.

The FOXA2 gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific genes such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. FOXA2 has been linked to sporadic cases of maturity-onset diabetes of the young. Transcript variants encoding different isoforms have been identified for FOXA2 (provided by RefSeq, Oct 2008).

Mouse anti Human FOXA2 antibody detects a band of 50 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species: Human
Western Blotting: Anti FOXA2 detects a band of approximately 50 kDa in HepG2 cell lysates.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Immunogen: Recombinant protein expressed in E.coli corresponding to amino acids 300-458 of human FOXA2

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Guarantee: 12 months from date of despatch

More Information

UniProt: Q9Y261
Entrez Gene: FOXA2
GO Terms: GO:0001701 in utero embryonic development; GO:0001705 ectoderm formation; GO:0003690 double-stranded DNA binding; GO:0003704 specific RNA polymerase II transcription factor activity; GO:0003705 sequence-specific enhancer binding RNA polymerase II transcription factor activity; GO:0005737 cytoplasm; GO:0005667 transcription factor complex; GO:0007492 endoderm development; GO:0008134 transcription factor binding; GO:0008301 DNA bending activity; GO:0009952 anterior/posterior pattern formation; GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter; GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter; GO:0010843 promoter binding; GO:0016563 transcription activator activity; GO:0016566 specific transcriptional repressor activity; GO:0016568 chromatin modification; GO:0019904 protein domain specific binding; GO:0021533 cell differentiation in hindbrain; GO:0021779 oligodendrocyte cell fate commitment; GO:0021904 dorsal/ventral neural tube patterning; GO:0030193 regulation of blood coagulation; GO:0031018 endocrine pancreas development; GO:0032289 central nervous system myelin formation; GO:0045665 negative regulation of neuron differentiation; GO:0045666 positive regulation of neuron differentiation; GO:0048665 neuron fate specification; GO:0051090 regulation of transcription factor activity; GO:0060441 epithelial tube branching involved in lung morphogenesis; GO:0060487 lung epithelial cell differentiation; GO:0070741 response to interleukin-6
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of FOXA2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in		Get Quote

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