FOXA1 antibody

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Mouse anti FOXA1

FOXA1 antibody recognizes FOXA1, also known as Hepatocyte nuclear factor 3-alpha, HNF-3-alpha, HNF-3A, TCF-3A or transcription factor 3A.

FOXA1 Antibody with Control Lysate

FOXA1 antibody recognizes FOXA1, also known as Hepatocyte nuclear factor 3-alpha, HNF-3-alpha, HNF-3A, TCF-3A or transcription factor 3A.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00663 WB 100 µl
VMA00663KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human FOXA1 antibody recognizes FOXA1, also known as Hepatocyte nuclear factor 3-alpha, HNF-3-alpha, HNF-3A, TCF-3A or transcription factor 3A.

FOXA1 encodes a member of the forkhead class of DNA-binding proteins.

FOXA1 encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver (provided by RefSeq, Jul 2008).

Mouse anti Human FOXA1 antibody detects a band of 50 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant human FOXA1 (aa 467-614)

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P55317 Related reagents
Entrez Gene
FOXA1 Related reagents
GO Terms
GO:0003690 double-stranded DNA binding
GO:0003704 specific RNA polymerase II transcription factor activity
GO:0003705 sequence-specific enhancer binding RNA polymerase II transcription factor activity
GO:0005667 transcription factor complex
GO:0006338 chromatin remodeling
GO:0009790 embryo development
GO:0007389 pattern specification process
GO:0008134 transcription factor binding
GO:0008301 DNA bending activity
GO:0008634 negative regulation of survival gene product expression
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010843 promoter binding
GO:0016563 transcription activator activity
GO:0016566 specific transcriptional repressor activity
GO:0019904 protein domain specific binding
GO:0032355 response to estradiol stimulus
GO:0033148 positive regulation of estrogen receptor signaling pathway
GO:0042593 glucose homeostasis
GO:0045666 positive regulation of neuron differentiation
GO:0045931 positive regulation of mitotic cell cycle
GO:0048665 neuron fate specification
GO:0051091 positive regulation of transcription factor activity
GO:0060441 epithelial tube branching involved in lung morphogenesis
GO:0060487 lung epithelial cell differentiation
GO:0060528 secretory columnal luminar epithelial cell differentiation involved in prostate glandular acinus development
GO:0060738 epithelial-mesenchymal signaling involved in prostate gland development
GO:0060740 prostate gland epithelium morphogenesis
GO:0060741 prostate gland stromal morphogenesis
GO:0060743 epithelial cell maturation involved in prostate gland development
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of FOXA1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Mouse anti FOXA1 antibody detects a band of approximately 50 kDa in HepG2 cell lysates
Western Blotting
Mouse anti FOXA1 antibody detects a band of approximately 50 kDa in HepG2 cell lysates
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB