Focal Adhesion Kinase antibody | OTI4A8

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Mouse anti Focal Adhesion Kinase

Product Type
PrecisionAb™ Monoclonal
Clone
OTI4A8
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00167
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human focal adhesion kinase 1 antibody recognizes focal adhesion kinase 1, also known as FADK 1, FAK-related non-kinase polypeptide, PTK2 protein tyrosine kinase 2, focal adhesion kinase 1, focal adhesion kinase isoform FAK-Del33, focal adhesion kinase-related nonkinase, protein phosphatase 1 regulatory subunit 71 and protein phosphatase 1, regulatory subunit 71.

Encoded by the PTK2 gene, focal adhesion kinase 1 is a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of focal adhesion kinase 1 may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for PTK2, but the full-length natures of only three of them have been determined (provided by RefSeq, Dec 2010).

Mouse anti Human focal adhesion kinase 1 antibody detects a band of 125 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti focal adhesion kinase detects a band of approximately 125 kDa in A549 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant protein of human focal adhesion kinase (NP_722560) produced in HEK293T cells

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
Q05397 Related reagents
Entrez Gene
PTK2 Related reagents
GO Terms
GO:0007596 blood coagulation
GO:0005829 cytosol
GO:0004715 non-membrane spanning protein tyrosine kinase activity
GO:0004871 signal transducer activity
GO:0005524 ATP binding
GO:0005856 cytoskeleton
GO:0005925 focal adhesion
GO:0006468 protein phosphorylation
GO:0006921 cellular component disassembly involved in apoptosis
GO:0007172 signal complex assembly
GO:0007229 integrin-mediated signaling pathway
GO:0007411 axon guidance
GO:0042169 SH2 domain binding
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Focal Adhesion Kinase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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