Focal Adhesion Kinase antibody | AbD05479
FAK plays a potential role in oncogenic transformation, and its expression is thought to be correlated to tumor invasiveness.
Product Details
- Target Species
- Human
- Product Form
- A monovalent human recombinant Fab selected from the HuCAL� GOLD phage display library, expressed in E. coli. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied liquid.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Full length recombinant human FAK expressed in Sf9 cells.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q05397
- Entrez Gene
- PTK2
- GO Terms
- GO:0007596 blood coagulation
- GO:0005829 cytosol
- GO:0004715 non-membrane spanning protein tyrosine kinase activity
- GO:0004871 signal transducer activity
- GO:0005524 ATP binding
- GO:0005856 cytoskeleton
- GO:0005925 focal adhesion
- GO:0006468 protein phosphorylation
- GO:0006921 cellular component disassembly involved in apoptosis
- GO:0007172 signal complex assembly
- GO:0007229 integrin-mediated signaling pathway
- GO:0007411 axon guidance
- GO:0042169 SH2 domain binding
- Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
His-tag is a registered trademark of EMD Biosciences. - Licensed Use
- For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
- Regulatory
- For research purposes only
Applications of Focal Adhesion Kinase antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | |||
Western Blotting | 1/100 | 1/1000 |
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- Western Blotting
- HCA065 detects a band of approximately 119 kDa in HCT-116 cell lysates
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse anti Histidine Tag:Alk.Phos. | MCA1396A | E WB | 0.1 mg |
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Mouse anti Histidine Tag:Biotin | MCA1396B | E WB | 0.1 mg |
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Mouse anti c-Myc:Alk.Phos. | MCA2200A | C E P WB* | 0.1 mg |
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Mouse anti c-Myc:Biotin | MCA2200B | C E P WB* | 0.1 mg |
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|
Mouse anti Penta Histidine Tag:HRP | MCA5995P | E WB | 125 µl |
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