Focal Adhesion Kinase antibody | AbD05479

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Human anti Focal Adhesion Kinase

Product Type
Monoclonal Antibody
Clone
AbD05479
Isotype
HuCAL Fab monovalent
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
HCA065 E WB 0.1 mg
Human anti focal adhesion kinase antibody, clone AbD05479 recognizes human focal adhesion kinase (FAK), a cytoplasmic protein tyrosine kinase of approximately 119 kDa that is activated by numerous stimuli and controls cell motility, proliferation and apoptosis. FAK is widely expressed in various tissues and cultured cells. It is located within focal adhesions that form between cells and plays a vital role in integrin signalling.

FAK plays a potential role in oncogenic transformation, and its expression is thought to be correlated to tumor invasiveness.

Product Details

Target Species
Human
Product Form
A monovalent human recombinant Fab selected from the HuCAL® GOLD phage display library, expressed in E. coli. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied liquid.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Full length recombinant human FAK expressed in Sf9 cells
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
Q05397 Related reagents
Entrez Gene
PTK2 Related reagents
GO Terms
GO:0007596 blood coagulation
GO:0005829 cytosol
GO:0004715 non-membrane spanning protein tyrosine kinase activity
GO:0004871 signal transducer activity
GO:0005524 ATP binding
GO:0005856 cytoskeleton
GO:0005925 focal adhesion
GO:0006468 protein phosphorylation
GO:0006921 cellular component disassembly involved in apoptosis
GO:0007172 signal complex assembly
GO:0007229 integrin-mediated signaling pathway
GO:0007411 axon guidance
GO:0042169 SH2 domain binding
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
His-tag is a registered trademark of EMD Biosciences.
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
Regulatory
For research purposes only

Applications of Focal Adhesion Kinase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting 1/100 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Western Blotting
HCA065 detects a band of approximately 119 kDa in HCT-116 cell lysates

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Mouse anti Histidine Tag:Alk.Phos. MCA1396A 0.1 mg E WB
Mouse anti Histidine Tag:Biotin MCA1396B 0.1 mg E WB
Mouse anti c-Myc:Alk.Phos. MCA2200A 0.1 mg C E P WB*
Mouse anti c-Myc:Biotin MCA2200B 0.1 mg C E P WB*
Mouse anti Penta Histidine Tag:HRP MCA5995P 125 µl E WB