FMR1 antibody | GH01/4C9
The protein encoded by FMR1 binds RNA and is associated with polysomes. The encoded protein may be involved in mRNA trafficking from the nucleus to the cytoplasm. A trinucleotide repeat (CGG) in the 5' UTR is normally found at 6-53 copies, but an expansion to 55-230 repeats is the cause of fragile X syndrome. Expansion of the trinucleotide repeat may also cause one form of premature ovarian failure (POF1). Multiple alternatively spliced transcript variants that encode different protein isoforms and which are located in different cellular locations have been described for FMR1 (provided by RefSeq, May 2010).
Mouse anti Human FMR1 antibody detects a band of 78 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
- Target Species
- Western Blotting
- Mouse anti FMR1 detects a band of approximately 78 kDa in HeLa cell lysates
- Species Cross-Reactivity
Target Species Cross Reactivity Mouse Rat
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - Liquid
- Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
- E. coli-derived recombinant protein of amino acids 36-279 of human FMR1
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- As supplied, 12 months from date of despatch
- Q06787 Related reagents
- Entrez Gene
- FMR1 Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0003729 mRNA binding
- GO:0005625 soluble fraction
- GO:0005737 cytoplasm
- GO:0005654 nucleoplasm
- GO:0005730 nucleolus
- GO:0005845 mRNA cap binding complex
- GO:0045947 negative regulation of translational initiation
- GO:0051028 mRNA transport
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- For research purposes only
Applications of FMR1 antibody
|Application Name||Verified||Min Dilution||Max Dilution|
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Secondary Antibodies Available
|Description||Product Code||Applications||Pack Size||List Price||Quantity|
|Goat anti Mouse IgG (H/L):HRP||STAR207P||WB||2 ml|
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