FMR1 antibody | GH01/4C9

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Mouse anti FMR1

Product Type
PrecisionAb Monoclonal
Clone
GH01/4C9
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00682
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human FMR1 antibody recognizes FMR1, also known as fragile X mental retardation protein 1.

The protein encoded by FMR1 binds RNA and is associated with polysomes. The encoded protein may be involved in mRNA trafficking from the nucleus to the cytoplasm. A trinucleotide repeat (CGG) in the 5' UTR is normally found at 6-53 copies, but an expansion to 55-230 repeats is the cause of fragile X syndrome. Expansion of the trinucleotide repeat may also cause one form of premature ovarian failure (POF1). Multiple alternatively spliced transcript variants that encode different protein isoforms and which are located in different cellular locations have been described for FMR1 (provided by RefSeq, May 2010).

Mouse anti Human FMR1 antibody detects a band of 78 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Mouse anti FMR1 detects a band of approximately 78 kDa in HeLa cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 36-279 of human FMR1
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
As supplied, 12 months from date of despatch

More Information

UniProt
Q06787 Related reagents
Entrez Gene
FMR1 Related reagents
GO Terms
GO:0005515 protein binding
GO:0003729 mRNA binding
GO:0005625 soluble fraction
GO:0005737 cytoplasm
GO:0005654 nucleoplasm
GO:0005730 nucleolus
GO:0005845 mRNA cap binding complex
GO:0045947 negative regulation of translational initiation
GO:0051028 mRNA transport
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of FMR1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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