Filamin-A antibody
Mouse anti Filamin-A
Filamin-A antibody recognizes filamin-A, also known as FLNA, actin binding protein 280, alpha-filamin, endothelial actin-binding protein, filamin-1 or non-muscle filamin. This antibody has been extensively validated for western blotting using whole cell lysates.
Filamin-A Antibody with Control Lysate
Filamin-A antibody recognizes filamin-A, also known as FLNA, actin binding protein 280, alpha-filamin, endothelial actin-binding protein, filamin-1 or non-muscle filamin. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00322 | WB | 100 µl | ||||
| VMA00322KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human filamin-A antibody recognizes filamin-A, also known as actin binding protein 280, alpha-filamin, endothelial actin-binding protein, filamin-1 and non-muscle filamin.
Filamin-A, encoded by FLNA gene, is an actin-binding protein that crosslinks actin filaments and links actin filaments to membrane glycoproteins. This protein is involved in remodeling the cytoskeleton to effect changes in cell shape and migration. Filamin-A interacts with integrins, transmembrane receptor complexes, and second messengers. Defects in the FLNA gene are a cause of several syndromes, including periventricular nodular heterotopias (PVNH1, PVNH4), otopalatodigital syndromes (OPD1, OPD2), frontometaphyseal dysplasia (FMD), Melnick-Needles syndrome (MNS), and X-linked congenital idiopathic intestinal pseudoobstruction (CIIPX). Two transcript variants encoding different isoforms have been found for FLNA (provided by RefSeq, Mar 2009).
Mouse anti Human filamin-A antibody detects a band of 280 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Filamin-A, encoded by FLNA gene, is an actin-binding protein that crosslinks actin filaments and links actin filaments to membrane glycoproteins. This protein is involved in remodeling the cytoskeleton to effect changes in cell shape and migration. Filamin-A interacts with integrins, transmembrane receptor complexes, and second messengers. Defects in the FLNA gene are a cause of several syndromes, including periventricular nodular heterotopias (PVNH1, PVNH4), otopalatodigital syndromes (OPD1, OPD2), frontometaphyseal dysplasia (FMD), Melnick-Needles syndrome (MNS), and X-linked congenital idiopathic intestinal pseudoobstruction (CIIPX). Two transcript variants encoding different isoforms have been found for FLNA (provided by RefSeq, Mar 2009).
Mouse anti Human filamin-A antibody detects a band of 280 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti filamin-A detects a band of approximately 280 kDa in HeLa cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
- Preparation
- 20μl Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Purified His-tagged filamin-A
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HeLa lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P21333 Related reagents
- Entrez Gene
- FLNA Related reagents
- GO Terms
- GO:0005886 plasma membrane
- GO:0001948 glycoprotein binding
- GO:0002576 platelet degranulation
- GO:0051015 actin filament binding
- GO:0005576 extracellular region
- GO:0005829 cytosol
- GO:0004871 signal transducer activity
- GO:0005634 nucleus
- GO:0005938 cell cortex
- GO:0007195 inhibition of adenylate cyclase activity by dopamine receptor signaling pathway
- GO:0008134 transcription factor binding
- GO:0015629 actin cytoskeleton
- GO:0050821 protein stabilization
- GO:0030168 platelet activation
- GO:0031532 actin cytoskeleton reorganization
- GO:0034329 cell junction assembly
- GO:0034394 protein localization at cell surface
- GO:0034988 Fc-gamma receptor I complex binding
- GO:0034989 GTP-Ral binding
- GO:0042177 negative regulation of protein catabolic process
- GO:0042803 protein homodimerization activity
- GO:0042993 positive regulation of transcription factor import into nucleus
- GO:0043113 receptor clustering
- GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
- GO:0043433 negative regulation of transcription factor activity
- GO:0045184 establishment of protein localization
- GO:0048365 Rac GTPase binding
- GO:0051220 cytoplasmic sequestering of protein
- GO:0051764 actin crosslink formation
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Filamin-A antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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