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Filamin-A antibody

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Anti Filamin-A Antibody (PrecisionAb Monoclonal Antibody) thumbnail image 1
Anti Filamin-A Antibody (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with filamin-A antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 8 second exposure. Arrow points to filamin-A (molecular weight 280 kDa)

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WB

Mouse anti Filamin-A

Product Type
PrecisionAb Monoclonal
Isotype
IgG1
Format
Purified
Specificity
Filamin-A

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VMA00322
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Mouse anti Human filamin-A antibody recognizes filamin-A, also known as actin binding protein 280, alpha-filamin, endothelial actin-binding protein, filamin-1 and non-muscle filamin.

Filamin-A, encoded by FLNA gene, is an actin-binding protein that crosslinks actin filaments and links actin filaments to membrane glycoproteins. This protein is involved in remodeling the cytoskeleton to effect changes in cell shape and migration. Filamin-A interacts with integrins, transmembrane receptor complexes, and second messengers. Defects in the FLNA gene are a cause of several syndromes, including periventricular nodular heterotopias (PVNH1, PVNH4), otopalatodigital syndromes (OPD1, OPD2), frontometaphyseal dysplasia (FMD), Melnick-Needles syndrome (MNS), and X-linked congenital idiopathic intestinal pseudoobstruction (CIIPX). Two transcript variants encoding different isoforms have been found for FLNA (provided by RefSeq, Mar 2009).

Mouse anti Human filamin-A antibody detects a band of 280 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti filamin-A detects a band of approximately 280 kDa in HeLa cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
Purified His-tagged filamin-A
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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Description Goat anti Mouse IgG (H/L):HRP

UniProt
P21333
Entrez Gene
FLNA
GO Terms
GO:0005886 plasma membrane
GO:0001948 glycoprotein binding
GO:0002576 platelet degranulation
GO:0051015 actin filament binding
GO:0005576 extracellular region
GO:0005829 cytosol
GO:0004871 signal transducer activity
GO:0005634 nucleus
GO:0005938 cell cortex
GO:0007195 inhibition of adenylate cyclase activity by dopamine receptor signaling pathway
GO:0008134 transcription factor binding
GO:0015629 actin cytoskeleton
GO:0050821 protein stabilization
GO:0030168 platelet activation
GO:0031532 actin cytoskeleton reorganization
GO:0034329 cell junction assembly
GO:0034394 protein localization at cell surface
GO:0034988 Fc-gamma receptor I complex binding
GO:0034989 GTP-Ral binding
GO:0042177 negative regulation of protein catabolic process
GO:0042803 protein homodimerization activity
GO:0042993 positive regulation of transcription factor import into nucleus
GO:0043113 receptor clustering
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0043433 negative regulation of transcription factor activity
GO:0045184 establishment of protein localization
GO:0048365 Rac GTPase binding
GO:0051220 cytoplasmic sequestering of protein
GO:0051764 actin crosslink formation
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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