FHL1 antibody | OTI2E11

Mouse anti FHL1

Product Type
PrecisionAb Monoclonal
Clone
OTI2E11
Isotype
IgG1
Format
Purified
Specificity
FHL1

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VMA00128
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WB 100 µl loader
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Mouse anti Human FHL1 antibody recognizes FHL1, also known as LIM protein SLIMMER, four and a half LIM domains protein 1, four-and-a-half Lin11, Isl-1 and Mec-3 domains 1 and skeletal muscle LIM-protein 1.

The FHL1 gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. Expression of these family members occurs in a cell- and tissue-specific mode and these proteins are involved in many cellular processes. Mutations in FHL1 have been found in patients with Emery-Dreifuss muscular dystrophy. Multiple alternately spliced transcript variants which encode different protein isoforms have been described (provided by RefSeq, Nov 2009).

Mouse anti Human FHL1 antibody detects a band of 32 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti FHL1 detects a band of approximately 32 kDa in HEK293 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant human FHL1 (NP_001440) produced in HEK293T cells
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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Description Goat anti Mouse IgG (H/L):HRP

UniProt
Q13642
Entrez Gene
FHL1
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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