Ferritin antibody | F31 (7B2)

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Anti Human Ferritin Antibody, clone F31 (7B2) thumbnail image 1

Anti Human Ferritin Antibody, clone F31 (7B2) thumbnail image 2

Anti Human Ferritin Antibody, clone F31 (7B2) gallery image 1 — Sandwich ELISA analysis of human ferritin using Mouse anti Human ferritin antibody, clone F31 (4420-3050) as a capture reagent and biotinylated Mouse anti Human ferritin antibody, clone 321B₇ (4420-3904) as a detection reagent with recombinant human ferritin (Bio-Rad Catalog no: 12000196) as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Normal serum (green) and plasma (orange) samples are included undiluted

Anti Human Ferritin Antibody, clone F31 (7B2) gallery image 2 — Sandwich ELISA analysis of human ferritin using Mouse anti Human ferritin antibody, clone F31 (4420-3050) as a capture reagent and biotinylated Mouse anti Human ferritin antibody, clone 321B₇ (4420-3904) as a detection reagent with purified human ferritin (4420-4804) as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Normal serum (green) and plasma (orange) samples are included undiluted

Mouse anti Human Ferritin

Product Type: Monoclonal Antibody
Clone: F31 (7B2)
Isotype: IgG2b

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
4420-3050	E			1 mg

Mouse anti Human ferritin antibody, clone F31 recognises human ferritin, an intracellular iron storage protein found in both eukaryotes and prokaryotes. Ferritin is a globular protein complex that binds iron, enabling storage in a soluble and non-toxic form.

Product Details

Target Species: Human
Product Form: Purified IgG - liquid.
Preparation: Purified IgG prepared by affinity chromatography on Protein G from ascites.
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Immunogen: Human ferritin, from liver.
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

More Information

UniProt: P02792 Related reagents; P02794 Related reagents
Entrez Gene: FTL Related reagents; FTH1 Related reagents
GO Terms: GO:0005829 cytosol; GO:0055114 oxidation-reduction process; GO:0016491 oxidoreductase activity; GO:0006826 iron ion transport; GO:0006879 cellular iron ion homeostasis; GO:0006892 post-Golgi vesicle-mediated transport; GO:0008043 intracellular ferritin complex; GO:0008199 ferric iron binding; GO:0042802 identical protein binding; GO:0016044 cellular membrane organization; GO:0005515 protein binding; GO:0004322 ferroxidase activity; GO:0006880 intracellular sequestering of iron ion; GO:0006955 immune response; GO:0008283 cell proliferation; GO:0008285 negative regulation of cell proliferation
Regulatory: For research purposes only

Applications of Ferritin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Human anti Mouse IgG2b:FITC	HCA038F	0.1 mg	F
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Useful Reagents Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Mouse anti Human Ferritin	4420-3010	1 mg	E

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