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Ferritin antibody | F31 (7B2)

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Anti Human Ferritin Antibody, clone F31 (7B2) thumbnail image 1 Anti Human Ferritin Antibody, clone F31 (7B2) thumbnail image 2
Anti Human Ferritin Antibody, clone F31 (7B2) gallery image 1
Sandwich ELISA analysis of human ferritin using Mouse anti Human ferritin antibody, clone F31 (4420-3050) as a capture reagent and biotinylated Mouse anti Human ferritin antibody, clone 321B7 (4420-3904) as a detection reagent with recombinant human ferritin (Bio-Rad Catalog no: 12000196) as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Normal serum (green) and plasma (orange) samples are included undiluted
Anti Human Ferritin Antibody, clone F31 (7B2) gallery image 2
Sandwich ELISA analysis of human ferritin using Mouse anti Human ferritin antibody, clone F31 (4420-3050) as a capture reagent and biotinylated Mouse anti Human ferritin antibody, clone 321B7 (4420-3904) as a detection reagent with purified human ferritin (4420-4804) as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Normal serum (green) and plasma (orange) samples are included undiluted

Mouse anti Human Ferritin

Product Type
Monoclonal Antibody
Clone
F31 (7B2)
Isotype
IgG2b
Product Code Applications Pack Size List Price Quantity
4420-3050
Datasheet
E
MSDS
1 mg loader

Mouse anti Human ferritin antibody, clone F31 recognises human ferritin, an intracellular iron storage protein found in both eukaryotes and prokaryotes. Ferritin is a globular protein complex that binds iron, enabling storage in a soluble and non-toxic form.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid.
Preparation
Purified IgG prepared by affinity chromatography on Protein G from ascites.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Human ferritin, from liver.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P02792
P02794
Entrez Gene
FTL
FTH1
GO Terms
GO:0005829 cytosol
GO:0055114 oxidation-reduction process
GO:0016491 oxidoreductase activity
GO:0006826 iron ion transport
GO:0006879 cellular iron ion homeostasis
GO:0006892 post-Golgi vesicle-mediated transport
GO:0008043 intracellular ferritin complex
GO:0008199 ferric iron binding
GO:0042802 identical protein binding
GO:0016044 cellular membrane organization
GO:0005515 protein binding
GO:0004322 ferroxidase activity
GO:0006880 intracellular sequestering of iron ion
GO:0006955 immune response
GO:0008283 cell proliferation
GO:0008285 negative regulation of cell proliferation
Regulatory
For research purposes only

Applications of Ferritin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Human Ferritin 4420-3010 E 1 mg loader
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