FAF1 antibody

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Mouse anti FAF1

Mouse anti human FAF1 antibody recognizes Fas associated factor 1, also known as UBX domain containing protein 12, UBX domain containing protein 3A or TNFRSF6 associated factor 1. This antibody has been extensively validated for western blotting using whole cell lysates.

FAF1 Antibody with Control Lysate

Product Type: PrecisionAb™ Monoclonal
Isotype: IgG2b
Format: Purified

Product Code	Applications	Pack Size
VMA00236	WB	100 µl
VMA00236KT	WB	2 Westerns
With Lysate		400µg

Summary
Secondary Antibodies

Mouse anti Human FAF1 antibody recognizes the FAS-associated factor 1, also known as TNFRSF6-associated factor 1, UBX domain protein 3A and UBX domain-containing protein 12.

Interaction of Fas ligand (TNFSF6) with the FAS antigen (TNFRSF6) mediates programmed cell death, also called apoptosis, in a number of organ systems. The protein encoded by FAF1 gene binds to FAS antigen and can initiate apoptosis or enhance apoptosis initiated through FAS antigen. Initiation of apoptosis by the protein encoded by FAF1 requires a ubiquitin-like domain but not the FAS-binding domain (provided by RefSeq, Jul 2008).

Mouse anti Human FAF1 antibody detects a band of 74 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species: Human
Western Blotting: Anti FAF1 detects a band of approximately 74 kDa in K562 cell lysates
Product Form: Purified IgG - liquid
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation: 20μl Mouse monoclonal antibody prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Immunogen: Recombinant human FAF1
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition: 400μg K562 lysate lyophilized in RIPA buffer.
Lysate Reconstitution: - If using DDT reconstitute the lyophilized lysate with 190μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Storage: Antibody: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life: As supplied, 12 months from date of despatch.
Shelf Life: As supplied, 12 months from date of despatch.

More Information

UniProt: Q9UNN5 Related reagents
Entrez Gene: FAF1 Related reagents
GO Terms: GO:0006915 apoptosis; GO:0005829 cytosol; GO:0007253 cytoplasmic sequestering of NF-kappaB; GO:0019887 protein kinase regulator activity; GO:0031072 heat shock protein binding; GO:0019901 protein kinase binding; GO:0031265 CD95 death-inducing signaling complex; GO:0031334 positive regulation of protein complex assembly; GO:0042176 regulation of protein catabolic process; GO:0043065 positive regulation of apoptosis; GO:0043161 proteasomal ubiquitin-dependent protein catabolic process; GO:0048471 perinuclear region of cytoplasm; GO:0051059 NF-kappaB binding
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of FAF1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB

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