FAF1 antibody

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Mouse anti FAF1

Mouse anti human FAF1 antibody recognizes Fas associated factor 1, also known as UBX domain containing protein 12, UBX domain containing protein 3A or TNFRSF6 associated factor 1. This antibody has been extensively validated for western blotting using whole cell lysates.

FAF1 Antibody with Control Lysate

Mouse anti human FAF1 antibody recognizes Fas associated factor 1, also known as UBX domain containing protein 12, UBX domain containing protein 3A or TNFRSF6 associated factor 1. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG2b
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00236 WB 100 µl
VMA00236KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human FAF1 antibody recognizes the FAS-associated factor 1, also known as TNFRSF6-associated factor 1, UBX domain protein 3A and UBX domain-containing protein 12.

Interaction of Fas ligand (TNFSF6) with the FAS antigen (TNFRSF6) mediates programmed cell death, also called apoptosis, in a number of organ systems. The protein encoded by FAF1 gene binds to FAS antigen and can initiate apoptosis or enhance apoptosis initiated through FAS antigen. Initiation of apoptosis by the protein encoded by FAF1 requires a ubiquitin-like domain but not the FAS-binding domain (provided by RefSeq, Jul 2008).

Mouse anti Human FAF1 antibody detects a band of 74 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti FAF1 detects a band of approximately 74 kDa in K562 cell lysates
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
20μl Mouse monoclonal antibody prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant human FAF1
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg K562 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
Q9UNN5 Related reagents
Entrez Gene
FAF1 Related reagents
GO Terms
GO:0006915 apoptosis
GO:0005829 cytosol
GO:0007253 cytoplasmic sequestering of NF-kappaB
GO:0019887 protein kinase regulator activity
GO:0031072 heat shock protein binding
GO:0019901 protein kinase binding
GO:0031265 CD95 death-inducing signaling complex
GO:0031334 positive regulation of protein complex assembly
GO:0042176 regulation of protein catabolic process
GO:0043065 positive regulation of apoptosis
GO:0043161 proteasomal ubiquitin-dependent protein catabolic process
GO:0048471 perinuclear region of cytoplasm
GO:0051059 NF-kappaB binding
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of FAF1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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