Ezrin antibody

Human anti Ezrin

Product Type
PrecisionAb Monoclonal
Isotype
HuCAL Fab bivalent
Format
Purified
Specificity
Ezrin

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VMA00447
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl loader
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Human anti Human ezrin antibody recognizes ezrin, otherwise known as p81 and villin 2. Ezrin is a 586 amino acid cytoplasmic peripheral membrane protein containing a single FERM domain and member of the ezrin, radixin, moesin (ERM) protein family, which acts as protein-tyrosine kinase substrate.

Ezrin is enriched in microvilli and other cell surface structures and plays a key role in cell surface structure adhesion, membrane trafficking, motility and organization (Ng et al. 2001). Ezrin acts as a linker protein between the plasma membrane and the actin cytoskeleton, and provides anchorage for cell surface molecules such as ICAM and CD44 (Finnerty et al. 2004). Ezrin is involved in the metastatic capacity of various types of human tumours including breast, prostate, bone, endometrial and colon cancers (Ohtani et al. 2002, Elzagheid et al. 2008).

Mouse anti Human ezrin antibody detects a band of 81 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti ezrin detects a band of approximately 81 kDa in HeLa cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is bivalent by dimerization of the bacterial alkaline phosphatase fusion protein. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Preparation
Human recombinant monoclonal antibody purified by StrepTactin affinity chromatography from E. coli extracts.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
His-tagged ezrin fusion protein
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the protein. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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UniProt
P15311
Entrez Gene
EZR
GO Terms
GO:0051015 actin filament binding
GO:0051017 actin filament bundle assembly
GO:0050839 cell adhesion molecule binding
GO:0005829 cytosol
GO:0005730 nucleolus
GO:0005884 actin filament
GO:0007016 cytoskeletal anchoring at plasma membrane
GO:0007159 leukocyte cell-cell adhesion
GO:0007411 axon guidance
GO:0008360 regulation of cell shape
GO:0016324 apical plasma membrane
GO:0019898 extrinsic to membrane
GO:0022614 membrane to membrane docking
GO:0030175 filopodium
GO:0030863 cortical cytoskeleton
GO:0031528 microvillus membrane
GO:0032587 ruffle membrane
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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