EZH2 antibody | OTI10B10

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Mouse anti EZH2

Product Type
PrecisionAb Monoclonal
Clone
OTI10B10
Isotype
IgG2b
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00276
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human EZH2 antibody recognizes histone-lysine N-methyltransferase EZH2, also known as enhancer of zeste homolog 2 and lysine N-methyltransferase 6.

Encoded by the EZH2 gene, histone-lysine N-methyltransferase EZH2 is a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for EZH2 (provided by RefSeq, Feb 2011).

Mouse anti Human EZH2 antibody detects a band of 98 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti EZH2 detects a band of approximately 98 kDa in Jurkat cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Recombinant protein corresponding to amino acids 182-431 of human EZH2 ( NP_004447) produced in E. coli

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
Q15910 Related reagents
Entrez Gene
EZH2 Related reagents
GO Terms
GO:0005515 protein binding
GO:0003677 DNA binding
GO:0006350 transcription
GO:0006355 regulation of transcription, DNA-dependent
GO:0016564 transcription repressor activity
GO:0018024 histone-lysine N-methyltransferase activity
GO:0035098 ESC/E(Z) complex
GO:0048387 negative regulation of retinoic acid receptor signaling pathway
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of EZH2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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