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EZH2 antibody | EF01/1C3.H6.1

Mouse anti EZH2

Product Type
PrecisionAb Monoclonal
Clone
EF01/1C3.H6.1
Isotype
IgG1
Format
Purified
Specificity
EZH2

Product Code Applications Pack Size List Price Your Price Qty
VMA00528
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl loader
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loader

Mouse anti Human EZH2 antibody recognizes the Histone-lysine N-methyltransferase EZH2, also known as enhancer of zeste homolog 2, or lysine N-methyltransferase 6.

The EZH2 gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for EZH2 (provided by RefSeq, Feb 2011).

Mouse anti Human EZH2 antibody detects a band of 105 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti EZH2 detects a band of approximately 105 kDa in Jurkat cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
E. coli-derived recombinant human EZH2 (amino acids 351 - 453)
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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Description Goat anti Mouse IgG (H/L):HRP

UniProt
Q15910
Entrez Gene
EZH2
GO Terms
GO:0005515 protein binding
GO:0003677 DNA binding
GO:0006350 transcription
GO:0006355 regulation of transcription, DNA-dependent
GO:0016564 transcription repressor activity
GO:0018024 histone-lysine N-methyltransferase activity
GO:0035098 ESC/E(Z) complex
GO:0048387 negative regulation of retinoic acid receptor signaling pathway

VMA00528

170420

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