EXPORTIN-1 antibody

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Mouse anti EXPORTIN-1

Exportin-1 antibody recognizes the exportin-1 protein, also known as CRM1 or chromosome region maintenance 1 homolog. This antibody has been extensively validated for western blotting using whole cell lysates.

EXPORTIN-1 Antibody with Control Lysate

Exportin-1 antibody recognizes the exportin-1 protein, also known as CRM1 or chromosome region maintenance 1 homolog. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG2b
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00085 WB 100 µl
VMA00085KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human exportin-1 antibody recognizes exportin-1, also known as Exp1, XPO1 and chromosome region maintenance 1 protein homolog.

Exportin-1 mediates leucine-rich nuclear export signal (NES)-dependent protein transport and specifically inhibits the nuclear export of Rev and U snRNAs. It is involved in the control of several cellular processes by controlling the localization of cyclin B, MPAK, and MAPKAP kinase 2. This protein also regulates NFAT and AP-1 (provided by RefSeq, Jul 2008).

Mouse anti Human exportin-1 antibody detects a band of 112 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti exportin-1 detects a band of approximately 112 kDa in Jurkat cell lysates.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
E. Coli derived recombinant protein, aa 932-1071 of human exportin-1
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg Jurkat lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
O14980 Related reagents
Entrez Gene
XPO1 Related reagents
GO Terms
GO:0005515 protein binding
GO:0000236 mitotic prometaphase
GO:0000776 kinetochore
GO:0003723 RNA binding
GO:0030529 ribonucleoprotein complex
GO:0005829 cytosol
GO:0005635 nuclear envelope
GO:0005642 annulate lamellae
GO:0005730 nucleolus
GO:0006886 intracellular protein transport
GO:0008565 protein transporter activity
GO:0015030 Cajal body
GO:0016071 mRNA metabolic process
GO:0019058 viral infectious cycle
GO:0046796 viral genome transport in host cell
GO:0051028 mRNA transport
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of EXPORTIN-1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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