ETS2 antibody | OTI3E10
Mouse anti ETS2
ETS2 antibody recognizes protein C-ets-2, also known as oncogene ETS-2 or v-ets avian erythroblastosis virus E2 oncogene homolog 2. This antibody has been extensively validated for western blotting using whole cell lysates.
ETS2 Antibody with Control Lysate
ETS2 antibody recognizes protein C-ets-2, also known as oncogene ETS-2 or v-ets avian erythroblastosis virus E2 oncogene homolog 2. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- OTI3E10
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00195 | WB | 100 µl | ||||
| VMA00195KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human ETS2 antibody recognizes ETS2, also known as protein C-ets-2, v-ets avian erythroblastosis virus E2 oncogene homolog 2 and v-ets erythroblastosis virus E26 oncogene homolog 2.
Encoded by the ETS2 gene, this protein is a transcription factor which regulates genes involved in development and apoptosis. It is also a protooncogene and has been shown to be involved in regulation of telomerase. A pseudogene of ETS2 is located on the X chromosome. Alternative splicing results in multiple transcript variants (provided by RefSeq, Jan 2012).
Mouse anti Human ETS2 antibody detects a band of 54 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Encoded by the ETS2 gene, this protein is a transcription factor which regulates genes involved in development and apoptosis. It is also a protooncogene and has been shown to be involved in regulation of telomerase. A pseudogene of ETS2 is located on the X chromosome. Alternative splicing results in multiple transcript variants (provided by RefSeq, Jan 2012).
Mouse anti Human ETS2 antibody detects a band of 54 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti ETS2 detects a band of approximately 54 kDa in HeLa cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography from ascites.
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatography from ascites.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- Recombinant protein corresponding to amino acids 90-349 of human ETS2 (NP_005230) produced in E. coli
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HeLa lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P15036 Related reagents
- Entrez Gene
- ETS2 Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0001501 skeletal system development
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0005634 nucleus
- GO:0045893 positive regulation of transcription, DNA-dependent
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of ETS2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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