Estrogen Sulfotransferase antibody

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Mouse anti Estrogen Sulfotransferase

Estrogen sulfotransferase antibody recognizes estrogen sulfotransferase also known as sulfotransferase 1E1, EST-1 or sulfotransferase, estrogen-preferring. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG2b
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00351
Datasheet
WB
MSDS
100 µl loader

Mouse anti estrogen sulfotransferase antibody detects estrogen sulfotransferase, also known as sulfotransferase 1E1, EST-1 and sulfotransferase, estrogen-preferring. Encoded by the SULT1E1 gene, the estrogen sulfotransferase enzyme is a member of a family that catalizes sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. This enzyme transfers a sulfo moiety to and from estrone, which may control levels of estrogen receptors.

Mouse anti human estrogen sulfotransferase antibody has been extensively validated for western blotting using whole cell lysates and detects a band of 35 kDa.

Product Details

Target Species
Human
Western Blotting
Anti estrogen sulfotransferase detects a band of approximately 35 kDa in HEK293 cell lysates
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant human estrogen sulfotransferase
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P49888 Related reagents
Entrez Gene
SULT1E1 Related reagents
GO Terms
GO:0004304 estrone sulfotransferase activity
GO:0005829 cytosol
GO:0005496 steroid binding
GO:0006805 xenobiotic metabolic process
GO:0031965 nuclear membrane
GO:0047894 flavonol 3-sulfotransferase activity
GO:0050294 steroid sulfotransferase activity
GO:0050427 3'-phosphoadenosine 5'-phosphosulfate metabolic process
GO:0051923 sulfation
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Estrogen Sulfotransferase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml
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