Erythropoietin antibody | KT28 (BGN/6B8)

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Mouse anti Human Erythropoietin

Product Type
Monoclonal Antibody
Clone
KT28 (BGN/6B8)
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
0300-0182 E 0.2 mg
Mouse anti Human erythropoietin antibody, clone KT28 recognizes erythropoietin (EPO) and binds to an epitope within the first 26 amino acids of the N-terminus of human urinary and human recombinant EPO.

EPO is a ~30 kDa glycoprotein hormone secreted primarily by the kidney that stimulates bone marrow erythropoiesis in response to hypoxia or anemia. The cDNAs for EPO have been cloned from many species. The mature proteins from the various species are highly conserved, exhibiting greater than 80% sequence identity at the amino acid level.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant human erythropoeitin.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P01588 Related reagents
Entrez Gene
EPO Related reagents
GO Terms
GO:0007165 signal transduction
GO:0005128 erythropoietin receptor binding
GO:0005179 hormone activity
GO:0005615 extracellular space
GO:0008015 blood circulation
GO:0008284 positive regulation of cell proliferation
GO:0042523 positive regulation of tyrosine phosphorylation of Stat5 protein
GO:0043193 positive regulation of gene-specific transcription
GO:0043249 erythrocyte maturation
GO:0045740 positive regulation of DNA replication
GO:0046579 positive regulation of Ras protein signal transduction
Regulatory
For research purposes only

Applications of Erythropoietin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/40K 1/160K
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.