Erythropoietin antibody | AE7A5

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Mouse anti Human Erythropoietin

Product Type
Monoclonal Antibody
Clone
AE7A5
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
4297-5005 WB 0.25 mg
Mouse anti Human Erythropoietin antibody, clone AE7A5 recognizes human erythropoietin (EPO) and binds to an epitope within the first 26 amino acids of the N-terminus of human urinary and human recombinant EPO.

EPO is a 166 amino acid ~30 kDa glycoprotein hormone secreted primarily by the kidney that stimulates bone marrow erythropoiesis in response to hypoxia or anemia. The cDNAs for Epo have been cloned from many species and the mature proteins from the various species are highly conserved, exhibiting greater than 80% sequence identity at the amino acid level.

Mouse anti Human Erythropoietin antibody, clone AE7A5 is the primary antibody sanctioned by the World Anti Doping Agency for the identification and evaluation of natural and recombinant erythropoietin by western blotting in biological samples (WADA doc: TD2014EPO). In addition to recognizing both recombinant and native human erythropoietin, clone AE7A5 cross reacts with zinc-alpha-2-glycoprotein, this reactivity is well separated from that of erythropoietin and does not interfere with the EPO testing assay (Reichel 2008).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Baboon
Dog
Sheep
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Immunogen
A 26 amino acid synthetic peptide corresponding to the N-terminal region of human erythropoietin with the sequence APPRLICDSRVLERYLLEAKEAENIT.
Approx. Protein Concentrations
1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P01588 Related reagents
Entrez Gene
EPO Related reagents
GO Terms
GO:0007165 signal transduction
GO:0005128 erythropoietin receptor binding
GO:0005179 hormone activity
GO:0005615 extracellular space
GO:0008015 blood circulation
GO:0008284 positive regulation of cell proliferation
GO:0042523 positive regulation of tyrosine phosphorylation of Stat5 protein
GO:0043193 positive regulation of gene-specific transcription
GO:0043249 erythrocyte maturation
GO:0045740 positive regulation of DNA replication
GO:0046579 positive regulation of Ras protein signal transduction
Regulatory
For research purposes only

Applications of Erythropoietin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F 0.1 mg F
Human anti Mouse IgG2a:HRP HCA037P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Application Based External Images

Western Blotting

Product Specific References

References for Erythropoietin antibody

  1. Sytkowski, A.J. and Fisher, J.W. (1985) Isolation and characterization of an anti-peptide monoclonal antibody to human erythropoietin.
    J Biol Chem. 260: 14727-31.
  2. Lasne, F. et al. (2009) Detection of continuous erythropoietin receptor activator in blood and urine in anti-doping control.
    Haematologica. 94: 888-90.
  3. Lasne, F. (2006) No doubt about the validity of the urine test for detection of recombinant human erythropoietin.
    Blood. 108 (5): 1778-9; author reply 1779-80.

Further Reading

  1. Sue, J.M. & Sytkowski, A.J. (1983) Site-specific antibodies to human erythropoietin directed toward the NH2-terminal region.
    Proc Natl Acad Sci U S A. 80 (12): 3651-5.