ERK1 antibody | 3D6

ISO 9001 Quality Assurance Manufacturing | Print

Anti Human ERK1 Antibody, clone 3D6 thumbnail image 1

Anti Human ERK1 Antibody, clone 3D6 gallery image 1 — Sandwich ELISA analysis of ERK1 binding using Mouse anti Human ERK1 antibody, clone 3C9 as a capture reagent and biotinylated Mouse anti Human ERK1 antibody, clone 3D6 (**MCA4808Z**) as a detection reagent with purified human ERK1 as antigen for the generation of the standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). A neat serum sample (green) is indicated

Filter by Application:

Mouse anti Human ERK1:Preservative Free

Product Type: Monoclonal Antibody
Clone: 3D6
Isotype: IgG2a
Specificity: ERK1

Quick Links:

Secondary Antibodies
Applications
Storage Information
Batch/Lot Specific Datasheets

Antibody Quality and Performance Guarantee

Unrivaled Technical Support

Product Code Applications Pack Size List Price Your Price Qty

List Price	Your Price
MCA4808Z Datasheet Datasheet SDS SDS	WB	0.1 mg		Log in	Get Quote
	Log in

Mouse anti Human ERK1 antibody, clone 3D6 recognizes Mitogen-activated protein kinase 3, also known as ERK1, Extracellular signal-regulated kinase 1 or p44-ERK1. ERK1 is a 379 amino acid ~44 kDa protein kinase. Three isoforms are generated by alternative splicing, clone 3D6 recognizes isoform 1, however it is not known whether the antibody also recognizes the other isoforms (UniProt: P27361).

Mouse anti Human ERK1 antibody, clone 3D6 has been sucessfully used as a detection reagent in the development of a sensitive Sandwich ELISA with Mouse anti Human ERK1 antibody, clone 3C9 as a capture reagent, with purified human ERK1 as antigen for the generation of the standard curve, for the detection of ERK1 in human samples.

Target Species: Human
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein A from ascites
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: None present
Immunogen: Recombinant protein corresponding to aa 279 - 379 of human ERK1 with a GST tag.
Approx. Protein Concentrations: Ig concentration 0.5 mg/ml
Fusion Partners: Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

Target Species	Cross Reactivity
Mouse
Rat

Store at -20^oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		0.1	10 ug/ml

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	C E P	0.5 mg	Log in	Get Quote
List Price	Your Price
	Log in
Description	Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	F	0.5 mg	Log in	Get Quote
List Price	Your Price
	Log in
Description	Goat anti Mouse IgG:FITC (Rat Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	F	1 ml	Log in	Get Quote
List Price	Your Price
	Log in
Description	Rabbit F(ab')2 anti Mouse IgG:RPE
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	F	1 ml	Log in	Get Quote
List Price	Your Price
	Log in
Description	Goat anti Mouse IgG:RPE (Rat Adsorbed)
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	E	1 mg	Log in	Get Quote
List Price	Your Price
	Log in
Description	Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	C E P RE WB	1 mg	Log in	Get Quote
List Price	Your Price
	Log in
Description	Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG (Fc):FITC	STAR120F	C F	1 mg	Log in	Get Quote
List Price	Your Price
	Log in
Description	Goat anti Mouse IgG (Fc):FITC
Goat anti Mouse IgG (Fc):HRP	STAR120P	E WB	1 mg	Log in	Get Quote
List Price	Your Price
	Log in
Description	Goat anti Mouse IgG (Fc):HRP
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	C E WB	0.5 mg	Log in	Get Quote
List Price	Your Price
	Log in
Description	Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	E WB	0.5 mg	Log in	Get Quote
List Price	Your Price
	Log in
Description	Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	F	0.5 mg	Log in	Get Quote
List Price	Your Price
	Log in
Description	Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	F IF	0.1 mg	Log in	Get Quote
List Price	Your Price
	Log in
Description	Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	F WB	0.1 mg	Log in	Get Quote
List Price	Your Price
	Log in
Description	Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	F IF WB	0.1 mg	Log in	Get Quote
List Price	Your Price
	Log in
Description	Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)	STAR117D650	F IF	0.1 mg	Log in	Get Quote
List Price	Your Price
	Log in
Description	Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)	STAR117D550	F IF WB	0.1 mg	Log in	Get Quote
List Price	Your Price
	Log in
Description	Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	C E WB	1 mg	Log in	Get Quote
List Price	Your Price
	Log in
Description	Goat anti Mouse IgG/A/M:Alk. Phos.
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	F	1 mg	Log in	Get Quote
List Price	Your Price
	Log in
Description	Rabbit F(ab')2 anti Mouse IgG:FITC

Synonyms: MAPK3
RRID: AB_2250301
UniProt: P27361
Entrez Gene: MAPK3
GO Terms: GO:0000186 activation of MAPKK activity; GO:0000187 activation of MAPK activity; GO:0002755 MyD88-dependent toll-like receptor signaling pathway; GO:0002756 MyD88-independent toll-like receptor signaling pathway; GO:0005829 cytosol; GO:0004707 MAP kinase activity; GO:0005524 ATP binding; GO:0005654 nucleoplasm; GO:0006361 transcription initiation from RNA polymerase I promoter; View More GO Terms; GO:0007049 cell cycle; GO:0007173 epidermal growth factor receptor signaling pathway; GO:0007265 Ras protein signal transduction; GO:0007411 axon guidance; GO:0008063 Toll signaling pathway; GO:0008286 insulin receptor signaling pathway; GO:0030168 platelet activation; GO:0034130 toll-like receptor 1 signaling pathway; GO:0034134 toll-like receptor 2 signaling pathway; GO:0034138 toll-like receptor 3 signaling pathway; GO:0034142 toll-like receptor 4 signaling pathway; GO:0044419 interspecies interaction between organisms; GO:0045087 innate immune response; GO:0048011 nerve growth factor receptor signaling pathway; GO:0051090 regulation of transcription factor activity; GO:0051403 stress-activated MAPK cascade