ERK1 antibody | 3D6
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|Mouse anti Human ERK1 antibody, clone 3D6 recognizes Mitogen-activated protein kinase 3, also known as ERK1, Extracellular signal-regulated kinase 1 or p44-ERK1. ERK1 is a 379 amino acid ~44 kDa protein kinase. Three isoforms are generated by alternative splicing, clone 3D6 recognizes isoform 1, however it is not known whether the antibody also recognizes the other isoforms (UniProt: P27361).
Mouse anti Human ERK1 antibody, clone 3D6 has been sucessfully used as a detection reagent in the development of a sensitive Sandwich ELISA with Mouse anti Human ERK1 antibody, clone 3C9 as a capture reagent, with purified human ERK1 as antigen for the generation of the standard curve, for the detection of ERK1 in human samples.
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Mouse Rat
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Recombinant protein corresponding to aa 279 - 379 of human ERK1 with a GST tag.
- Approx. Protein Concentrations
- Ig concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
- For research purposes only
- 12 months from date of despatch
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
|Application Name||Verified||Min Dilution||Max Dilution|
|Western Blotting||0.1||10 ug/ml|
- Entrez Gene
- GO Terms
- GO:0000186 activation of MAPKK activity
- GO:0000187 activation of MAPK activity
- GO:0002755 MyD88-dependent toll-like receptor signaling pathway
- GO:0002756 MyD88-independent toll-like receptor signaling pathway
- GO:0005829 cytosol
- GO:0004707 MAP kinase activity
- GO:0005524 ATP binding
- GO:0005654 nucleoplasm
- GO:0006361 transcription initiation from RNA polymerase I promoter
- View More GO Terms
- GO:0007049 cell cycle
- GO:0007173 epidermal growth factor receptor signaling pathway
- GO:0007265 Ras protein signal transduction
- GO:0007411 axon guidance
- GO:0008063 Toll signaling pathway
- GO:0008286 insulin receptor signaling pathway
- GO:0030168 platelet activation
- GO:0034130 toll-like receptor 1 signaling pathway
- GO:0034134 toll-like receptor 2 signaling pathway
- GO:0034138 toll-like receptor 3 signaling pathway
- GO:0034142 toll-like receptor 4 signaling pathway
- GO:0044419 interspecies interaction between organisms
- GO:0045087 innate immune response
- GO:0048011 nerve growth factor receptor signaling pathway
- GO:0051090 regulation of transcription factor activity
- GO:0051403 stress-activated MAPK cascade
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