ERK1 antibody | 3D6
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E ResetMouse anti Human ERK1:Preservative Free
- Product Type
- Monoclonal Antibody
- Clone
- 3D6
- Isotype
- IgG2a
- Specificity
- ERK1
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| Mouse anti Human ERK1 antibody, clone 3D6 recognizes Mitogen-activated protein kinase 3, also known as ERK1, Extracellular signal-regulated kinase 1 or p44-ERK1. ERK1 is a 379 amino acid ~44 kDa protein kinase. Three isoforms are generated by alternative splicing, clone 3D6 recognizes isoform 1, however it is not known whether the antibody also recognizes the other isoforms (UniProt: P27361). Mouse anti Human ERK1 antibody, clone 3D6 has been sucessfully used as a detection reagent in the development of a sensitive Sandwich ELISA with Mouse anti Human ERK1 antibody, clone 3C9 as a capture reagent, with purified human ERK1 as antigen for the generation of the standard curve, for the detection of ERK1 in human samples. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Immunogen
- Recombinant protein corresponding to aa 279 - 379 of human ERK1 with a GST tag.
- Approx. Protein Concentrations
- Ig concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting |  |
0.1 | 10 ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Synonyms
- MAPK3
- RRID
- AB_2250301
- UniProt
- P27361
- Entrez Gene
- MAPK3
- GO Terms
- GO:0000186 activation of MAPKK activity
- GO:0000187 activation of MAPK activity
- GO:0002755 MyD88-dependent toll-like receptor signaling pathway
- GO:0002756 MyD88-independent toll-like receptor signaling pathway
- GO:0005829 cytosol
- GO:0004707 MAP kinase activity
- GO:0005524 ATP binding
- GO:0005654 nucleoplasm
- GO:0006361 transcription initiation from RNA polymerase I promoter
- View More GO Terms
- GO:0007049 cell cycle
- GO:0007173 epidermal growth factor receptor signaling pathway
- GO:0007265 Ras protein signal transduction
- GO:0007411 axon guidance
- GO:0008063 Toll signaling pathway
- GO:0008286 insulin receptor signaling pathway
- GO:0030168 platelet activation
- GO:0034130 toll-like receptor 1 signaling pathway
- GO:0034134 toll-like receptor 2 signaling pathway
- GO:0034138 toll-like receptor 3 signaling pathway
- GO:0034142 toll-like receptor 4 signaling pathway
- GO:0044419 interspecies interaction between organisms
- GO:0045087 innate immune response
- GO:0048011 nerve growth factor receptor signaling pathway
- GO:0051090 regulation of transcription factor activity
- GO:0051403 stress-activated MAPK cascade
View more products with ERK specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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