ERK1/2 antibody

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Mouse anti ERK1/2

<strong>Mouse anti-human ERK1/2 antibody</strong> recognizes mitogen-activated protein kinase 3/1. This antibody has been extensively validated for western blotting using whole cell lysates.

ERK1/2 Antibody with Control Lysate

<strong>Mouse anti-human ERK1/2 antibody</strong> recognizes mitogen-activated protein kinase 3/1. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type: PrecisionAb™ Monoclonal
Isotype: IgG2a

Product Code	Applications	Pack Size
VMA00485	WB	100 µl
VMA00485KT	WB	2 Westerns
With Lysate		400µg

Summary
Secondary Antibodies

Mouse anti Human ERK1/2 antibody recognizes ERK1/2, also known as MAPK3/1.

MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. ERK1/2 are proline-directed kinases that preferentially catalyze the phosphorylation of substrates containing a Pro-Xxx-Ser/Thr-Pro sequence. Alternatively spliced transcript variants encoding different protein isoforms have been described (provided by RefSeq, Jul 2008).

Mouse anti Human ERK1/2 antibody detects bands of 42 and 44 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Product Form

Purified IgG - liquid

Preparation

Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant

Preparation

20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)

Immunogen

Purified His-tagged ERK 1/2 protein

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Storage: Antibody: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life: As supplied, 12 months from date of despatch.
Shelf Life: As supplied, 12 months from date of despatch.

More Information

UniProt: P27361 Related reagents; P28482 Related reagents
Entrez Gene: MAPK3 Related reagents; MAPK1 Related reagents
GO Terms: GO:0000186 activation of MAPKK activity; GO:0000187 activation of MAPK activity; GO:0002755 MyD88-dependent toll-like receptor signaling pathway; GO:0002756 MyD88-independent toll-like receptor signaling pathway; GO:0005829 cytosol; GO:0004707 MAP kinase activity; GO:0005524 ATP binding; GO:0005654 nucleoplasm; GO:0006361 transcription initiation from RNA polymerase I promoter; GO:0007049 cell cycle; GO:0007173 epidermal growth factor receptor signaling pathway; GO:0007265 Ras protein signal transduction; GO:0007411 axon guidance; GO:0008063 Toll signaling pathway; GO:0008286 insulin receptor signaling pathway; GO:0030168 platelet activation; GO:0034130 toll-like receptor 1 signaling pathway; GO:0034134 toll-like receptor 2 signaling pathway; GO:0034138 toll-like receptor 3 signaling pathway; GO:0034142 toll-like receptor 4 signaling pathway; GO:0044419 interspecies interaction between organisms; GO:0045087 innate immune response; GO:0048011 nerve growth factor receptor signaling pathway; GO:0051090 regulation of transcription factor activity; GO:0051403 stress-activated MAPK cascade; GO:0003677 DNA binding; GO:0006350 transcription; GO:0006917 induction of apoptosis; GO:0007268 synaptic transmission; GO:0008353 RNA polymerase II carboxy-terminal domain kinase activity; GO:0070371 ERK1 and ERK2 cascade
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of ERK1/2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Western Blotting: Anti ERK1/2 detects band of approximately 42 and 44 kDa in NIH/3T3 cell lysate
Western Blotting: Anti ERK1/2 detects bands of approximately 42 and 44 kDa in NIH/3T3 cell lysate
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB

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