ERCC1 antibody | OTI1H1

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Anti ERCC1 Antibody, clone OTI1H1 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti ERCC1 Antibody, clone OTI1H1 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with ERCC1 antibody followed by detection with Goat anti Mouse HRP conjugated (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 8 second exposure. Arrow points to ERCC1 (molecular weight 39 kDa)

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Mouse anti ERCC1

Product Type: PrecisionAb Monoclonal
Clone: OTI1H1
Isotype: IgG2a
Format: Purified
Specificity: ERCC1

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VMA00598 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti Human ERCC1 antibody recognizes the DNA excision repair protein ERCC-1, also known as excision repair cross-complementing rodent repair deficiency complementation group 1.

The product of ERCC1 functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in ERCC1 result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of ERCC1 may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for ERCC1. The last exon of ERCC1 overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand (provided by RefSeq, Oct 2009).

Mouse anti Human ERCC1 antibody detects a band of 39 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Mouse anti ERCC1 antibody detects a band of approximately 39 kDa in MCF7 cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatograpy on Protein A from ascites.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
<50% Glycerol
Immunogen: HEK293-derived full length recombinant human ERCC1
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: P07992
Entrez Gene: ERCC1
GO Terms: GO:0000109 nucleotide-excision repair complex; GO:0000718 nucleotide-excision repair, DNA damage removal; GO:0000784 nuclear chromosome, telomeric region; GO:0003684 damaged DNA binding; GO:0003697 single-stranded DNA binding; GO:0004519 endonuclease activity; GO:0005737 cytoplasm; GO:0005654 nucleoplasm; GO:0006283 transcription-coupled nucleotide-excision repair; View More GO Terms; GO:0006295 nucleotide-excision repair, DNA incision, 3'-to lesion; GO:0006296 nucleotide-excision repair, DNA incision, 5'-to lesion; GO:0006312 mitotic recombination; GO:0006979 response to oxidative stress; GO:0008022 protein C-terminus binding; GO:0019904 protein domain specific binding; GO:0032205 negative regulation of telomere maintenance