ERCC1 antibody | OTI1H1

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Mouse anti ERCC1

ERCC1 antibody recognizes the DNA excision repair protein ERCC-1, also known as excision repair cross-complementing rodent repair deficiency complementation group 1.

ERCC1 Antibody with Control Lysate

ERCC1 antibody recognizes the DNA excision repair protein ERCC-1, also known as excision repair cross-complementing rodent repair deficiency complementation group 1.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI1H1
Isotype
IgG2a
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00598 WB 100 µl
VMA00598KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human ERCC1 antibody recognizes the DNA excision repair protein ERCC-1, also known as excision repair cross-complementing rodent repair deficiency complementation group 1.

The product of ERCC1 functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in ERCC1 result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of ERCC1 may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for ERCC1. The last exon of ERCC1 overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand (provided by RefSeq, Oct 2009).

Mouse anti Human ERCC1 antibody detects a band of 39 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Mouse anti ERCC1 antibody detects a band of approximately 39 kDa in MCF7 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatograpy on Protein A from ascites
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatograpy on Protein A from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
<50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
<50% Glycerol
Immunogen
HEK293-derived full length recombinant human ERCC1
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg MCF7 lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P07992 Related reagents
Entrez Gene
ERCC1 Related reagents
GO Terms
GO:0000109 nucleotide-excision repair complex
GO:0000718 nucleotide-excision repair, DNA damage removal
GO:0000784 nuclear chromosome, telomeric region
GO:0003684 damaged DNA binding
GO:0003697 single-stranded DNA binding
GO:0004519 endonuclease activity
GO:0005737 cytoplasm
GO:0005654 nucleoplasm
GO:0006283 transcription-coupled nucleotide-excision repair
GO:0006295 nucleotide-excision repair, DNA incision, 3'-to lesion
GO:0006296 nucleotide-excision repair, DNA incision, 5'-to lesion
GO:0006312 mitotic recombination
GO:0006979 response to oxidative stress
GO:0008022 protein C-terminus binding
GO:0019904 protein domain specific binding
GO:0032205 negative regulation of telomere maintenance
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of ERCC1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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