ERBB2 (pTyr1112) antibody
Learn more about TPN Total Protein Normalization).
Mouse anti Human ErbB2 antibody recognizes receptor tyrosine-protein kinase erbB2, also known as proto-oncogene c-ErbB-2, receptor tyrosine-protein kinase ErbB-2, tyrosine kinase-type cell surface receptor HER2, when phosphorylated at tyrosine 1112. It belongs to the ErbB family of receptor tyrosine kinases (Muthuswamy et al. 1999), which plays an important roles in the development of organs such as the mammary gland (Xie et al. 1997) and central nervous system (Carraway 1996). Phosphorylated at tyrosine 1112 provides a docking site for c-Cbl E3 ligase, which mediates receptor ubiquitination (Hynes and Stern 1994). PTPN18 dephosphorylates ErbB2 on tyrosine 1112 thereby blocking the lysosomal routing of the receptor tyrosine kinase (Wang et al. 2014).
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti ErbB2 (pTyr1112) detects a band of approximately 185 kDa in HepG2 + EGF (treated lysate) cell lysates
- Product Form
- Purified IgG - Liquid
- Preparation
- Mouse monoclonal antibody purified by ultrafiltration and size exclusion chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
PEG & Sucrose - Immunogen
- Phosphopeptide conjugated to hemocyanin
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P04626
- Entrez Gene
- ERBB2
- GO Terms
- GO:0003677 DNA binding
- GO:0016021 integral to membrane
- GO:0004716 receptor signaling protein tyrosine kinase activity
- GO:0005006 epidermal growth factor receptor activity
- GO:0043125 ErbB-3 class receptor binding
- GO:0005524 ATP binding
- GO:0005634 nucleus
- GO:0006350 transcription
- GO:0007507 heart development
- GO:0008022 protein C-terminus binding
- GO:0008283 cell proliferation
- GO:0042802 identical protein binding
- GO:0014065 phosphatidylinositol 3-kinase cascade
- GO:0019903 protein phosphatase binding
- GO:0048015 phosphatidylinositol-mediated signaling
- GO:0042060 wound healing
- GO:0043235 receptor complex
- GO:0043406 positive regulation of MAP kinase activity
- GO:0045449 regulation of transcription
- GO:0045765 regulation of angiogenesis
- GO:0045785 positive regulation of cell adhesion
- GO:0046777 protein autophosphorylation
- GO:0046982 protein heterodimerization activity
- GO:0048471 perinuclear region of cytoplasm
- GO:0050679 positive regulation of epithelial cell proliferation
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of ERBB2 (pTyr1112) antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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