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ERBB2 (pTyr1112) antibody

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Western Blot Validated
Anti ErbB2 (pTyr1112) Antibody (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti ErbB2 (pTyr1112) Antibody (PrecisionAb Monoclonal Antibody) thumbnail image 2
Anti ErbB2 (pTyr1112) Antibody (PrecisionAb Monoclonal Antibody) gallery image 1
Western Blot analysis of HepG2 untreated and EGF treated whole cell lysates probed with (A) Mouse anti ErbB2 antibody (VMA00147) or (B) Mouse anti ErbB2 (pTyr1112) antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P). Membranes were treated with (+) and without (-) lambda protein phosphatase and visualized on the ChemiDoc MP with a (A) 51 second or a (B) 10 second exposure. ErbB2 (pTyr1112) has a molecular weight of 185 kDa.
Anti ErbB2 (pTyr1112) Antibody (PrecisionAb Monoclonal Antibody) gallery image 2
Normalized signal intensity of ErbB2 (pTyr1112) from EGF treated and untreated lysates, treated with (+) or without (-) lambda protein phosphatase. Lambda protein phosphatase dephosphorylates serine, threonine and tyrosine residues. Normalized signal intensity was calculated by dividing the TPN-Normalized signal intensity for each phosphoprotein by the TPN-normalized intensity of the corresponding total protein.
Learn more about TPN Total Protein Normalization).

Mouse anti ErbB2 (pTyr1112)

Product Type
PrecisionAb Monoclonal
Isotype
IgG1
Format
Purified
PrecisionAb Antibodies
Product Code Applications Pack Size List Price Quantity
VMA00753
Datasheet
WB
SDS
100 µl loader

Mouse anti Human ErbB2 antibody recognizes receptor tyrosine-protein kinase erbB2, also known as proto-oncogene c-ErbB-2, receptor tyrosine-protein kinase ErbB-2, tyrosine kinase-type cell surface receptor HER2, when phosphorylated at tyrosine 1112. It belongs to the ErbB family of receptor tyrosine kinases (Muthuswamy et al. 1999), which plays an important roles in the development of organs such as the mammary gland (Xie et al. 1997) and central nervous system (Carraway 1996). Phosphorylated at tyrosine 1112 provides a docking site for c-Cbl E3 ligase, which mediates receptor ubiquitination (Hynes and Stern 1994). PTPN18 dephosphorylates ErbB2 on tyrosine 1112 thereby blocking the lysosomal routing of the receptor tyrosine kinase (Wang et al. 2014).

Product Details

Target Species
Human
Western Blotting
Mouse anti ErbB2 (pTyr1112) detects a band of approximately 185 kDa in HepG2 + EGF (treated lysate) cell lysates
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody purified by ultrafiltration and size exclusion chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
PEG & Sucrose
Immunogen
Phosphopeptide conjugated to hemocyanin

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P04626
Entrez Gene
ERBB2
GO Terms
GO:0003677 DNA binding
GO:0016021 integral to membrane
GO:0004716 receptor signaling protein tyrosine kinase activity
GO:0005006 epidermal growth factor receptor activity
GO:0043125 ErbB-3 class receptor binding
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0006350 transcription
GO:0007507 heart development
GO:0008022 protein C-terminus binding
GO:0008283 cell proliferation
GO:0042802 identical protein binding
GO:0014065 phosphatidylinositol 3-kinase cascade
GO:0019903 protein phosphatase binding
GO:0048015 phosphatidylinositol-mediated signaling
GO:0042060 wound healing
GO:0043235 receptor complex
GO:0043406 positive regulation of MAP kinase activity
GO:0045449 regulation of transcription
GO:0045765 regulation of angiogenesis
GO:0045785 positive regulation of cell adhesion
GO:0046777 protein autophosphorylation
GO:0046982 protein heterodimerization activity
GO:0048471 perinuclear region of cytoplasm
GO:0050679 positive regulation of epithelial cell proliferation
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of ERBB2 (pTyr1112) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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