ERBB2 (pTyr1112) antibody

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Anti ErbB2 (pTyr1112) Antibody (PrecisionAb™ Monoclonal Antibody) thumbnail image 2

Anti ErbB2 (pTyr1112) Antibody (PrecisionAb™ Monoclonal Antibody) gallery image 1 — Western Blot analysis of HepG2 untreated and EGF treated whole cell lysates probed with (A) Mouse anti ErbB2 antibody (VMA00147) or (B) Mouse anti ErbB2 (pTyr1112) antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P). Membranes were treated with (+) and without (-) lambda protein phosphatase and visualized on the ChemiDoc MP with a (A) 51 second or a (B) 10 second exposure. ErbB2 (pTyr1112) has a molecular weight of 185 kDa.

Anti ErbB2 (pTyr1112) Antibody (PrecisionAb™ Monoclonal Antibody) gallery image 2 — Normalized signal intensity of ErbB2 (pTyr1112) from EGF treated and untreated lysates, treated with (+) or without (-) lambda protein phosphatase. Lambda protein phosphatase dephosphorylates serine, threonine and tyrosine residues. Normalized signal intensity was calculated by dividing the TPN-Normalized signal intensity for each phosphoprotein by the TPN-normalized intensity of the corresponding total protein.
Learn more about TPN Total Protein Normalization).

Mouse anti ErbB2 (pTyr1112)

ErbB2 (pTyr1112) antibody recognizes ErbB2, also known as c-erb B2/neu protein, metastatic lymph node gene 19 protein phosphorylated at Tyr1112.

Product Type: PrecisionAb™ Monoclonal
Isotype: IgG1
Format: Purified

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
VMA00753	WB			100 µl

Summary
Secondary Antibodies

Mouse anti Human ErbB2 antibody recognizes receptor tyrosine-protein kinase erbB2, also known as proto-oncogene c-ErbB-2, receptor tyrosine-protein kinase ErbB-2, tyrosine kinase-type cell surface receptor HER2, when phosphorylated at tyrosine 1112. It belongs to the ErbB family of receptor tyrosine kinases (Muthuswamy et al. 1999), which plays an important roles in the development of organs such as the mammary gland (Xie et al. 1997) and central nervous system (Carraway 1996). Phosphorylated at tyrosine 1112 provides a docking site for c-Cbl E3 ligase, which mediates receptor ubiquitination (Hynes and Stern 1994). PTPN18 dephosphorylates ErbB2 on tyrosine 1112 thereby blocking the lysosomal routing of the receptor tyrosine kinase (Wang et al. 2014).

Product Details

Target Species: Human
Western Blotting: Mouse anti ErbB2 (pTyr1112) detects a band of approximately 185 kDa in HepG2 + EGF (treated lysate) cell lysates
Product Form: Purified IgG - Liquid
Preparation: Mouse monoclonal antibody purified by ultrafiltration and size exclusion chromatography from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide
PEG & Sucrose
Immunogen: Phosphopeptide conjugated to hemocyanin
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage: Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Shelf Life: As supplied, 12 months from date of despatch

More Information

UniProt: P04626 Related reagents
Entrez Gene: ERBB2 Related reagents
GO Terms: GO:0003677 DNA binding; GO:0016021 integral to membrane; GO:0004716 receptor signaling protein tyrosine kinase activity; GO:0005006 epidermal growth factor receptor activity; GO:0043125 ErbB-3 class receptor binding; GO:0005524 ATP binding; GO:0005634 nucleus; GO:0006350 transcription; GO:0007507 heart development; GO:0008022 protein C-terminus binding; GO:0008283 cell proliferation; GO:0042802 identical protein binding; GO:0014065 phosphatidylinositol 3-kinase cascade; GO:0019903 protein phosphatase binding; GO:0048015 phosphatidylinositol-mediated signaling; GO:0042060 wound healing; GO:0043235 receptor complex; GO:0043406 positive regulation of MAP kinase activity; GO:0045449 regulation of transcription; GO:0045765 regulation of angiogenesis; GO:0045785 positive regulation of cell adhesion; GO:0046777 protein autophosphorylation; GO:0046982 protein heterodimerization activity; GO:0048471 perinuclear region of cytoplasm; GO:0050679 positive regulation of epithelial cell proliferation
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory: For research purposes only

Applications of ERBB2 (pTyr1112) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB

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