ERBB2 (pTyr1112) antibody

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Mouse anti ErbB2 (pTyr1112)

ErbB2 (pTyr1112) antibody recognizes ErbB2, also known as c-erb B2/neu protein, metastatic lymph node gene 19 protein phosphorylated at Tyr1112.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Format
Purified
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00753 WB 100 µl
Mouse anti Human ErbB2 antibody recognizes receptor tyrosine-protein kinase erbB2, also known as proto-oncogene c-ErbB-2, receptor tyrosine-protein kinase ErbB-2, tyrosine kinase-type cell surface receptor HER2, when phosphorylated at tyrosine 1112. It belongs to the ErbB family of receptor tyrosine kinases (Muthuswamy et al. 1999), which plays an important roles in the development of organs such as the mammary gland (Xie et al. 1997) and central nervous system (Carraway 1996). Phosphorylated at tyrosine 1112 provides a docking site for c-Cbl E3 ligase, which mediates receptor ubiquitination (Hynes and Stern 1994). PTPN18 dephosphorylates ErbB2 on tyrosine 1112 thereby blocking the lysosomal routing of the receptor tyrosine kinase (Wang et al. 2014).

Product Details

Target Species
Human
Western Blotting
Mouse anti ErbB2 (pTyr1112) detects a band of approximately 185 kDa in HepG2 + EGF (treated lysate) cell lysates
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody purified by ultrafiltration and size exclusion chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
PEG & Sucrose
Immunogen
Phosphopeptide conjugated to hemocyanin
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P04626 Related reagents
Entrez Gene
ERBB2 Related reagents
GO Terms
GO:0003677 DNA binding
GO:0016021 integral to membrane
GO:0004716 receptor signaling protein tyrosine kinase activity
GO:0005006 epidermal growth factor receptor activity
GO:0043125 ErbB-3 class receptor binding
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0006350 transcription
GO:0007507 heart development
GO:0008022 protein C-terminus binding
GO:0008283 cell proliferation
GO:0042802 identical protein binding
GO:0014065 phosphatidylinositol 3-kinase cascade
GO:0019903 protein phosphatase binding
GO:0048015 phosphatidylinositol-mediated signaling
GO:0042060 wound healing
GO:0043235 receptor complex
GO:0043406 positive regulation of MAP kinase activity
GO:0045449 regulation of transcription
GO:0045765 regulation of angiogenesis
GO:0045785 positive regulation of cell adhesion
GO:0046777 protein autophosphorylation
GO:0046982 protein heterodimerization activity
GO:0048471 perinuclear region of cytoplasm
GO:0050679 positive regulation of epithelial cell proliferation
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of ERBB2 (pTyr1112) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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