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ErbB2 antibody | AB04/4D7

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Anti ErbB2 Antibody, clone AB04/4D7 (PrecisionAb Monoclonal Antibody) thumbnail image 1
Anti ErbB2 Antibody, clone AB04/4D7 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with ErbB2 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 72 second exposure. Arrow points to ErbB2 (molecular weight 185 kDa)

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WB

Mouse anti ErbB2

Product Type
PrecisionAb Monoclonal
Clone
AB04/4D7
Isotype
IgG1
Format
Purified
Specificity
ErbB2

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Mouse anti Human ErbB2 antibody recognizes ErbB2 also known as receptor tyrosine-protein kinase erbB-2 or herstatin.

The ERBB2 gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized (provided by RefSeq, Jul 2008)

Mouse anti Human ErbB2 antibody detects a band of 185 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.		 PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti ErbB2 detects a band of approximately 185 kDa in SK-OV-3 cell lysates.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
E. coli-derived recombinant human ErbB2 (amino acids 22 - 254)
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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Description Goat anti Mouse IgG (H/L):HRP

UniProt
P04626
Entrez Gene
ERBB2
GO Terms
GO:0003677 DNA binding
GO:0016021 integral to membrane
GO:0004716 receptor signaling protein tyrosine kinase activity
GO:0005006 epidermal growth factor receptor activity
GO:0043125 ErbB-3 class receptor binding
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0006350 transcription
GO:0007507 heart development
GO:0008022 protein C-terminus binding
GO:0008283 cell proliferation
GO:0042802 identical protein binding
GO:0014065 phosphatidylinositol 3-kinase cascade
GO:0019903 protein phosphatase binding
GO:0048015 phosphatidylinositol-mediated signaling
GO:0042060 wound healing
GO:0043235 receptor complex
GO:0043406 positive regulation of MAP kinase activity
GO:0045449 regulation of transcription
GO:0045765 regulation of angiogenesis
GO:0045785 positive regulation of cell adhesion
GO:0046777 protein autophosphorylation
GO:0046982 protein heterodimerization activity
GO:0048471 perinuclear region of cytoplasm
GO:0050679 positive regulation of epithelial cell proliferation
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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