ERAB antibody | OTI5E2

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Mouse anti ERAB

Mouse anti human ERAB antibody recognizes 3-hydroxyacyl-CoA dehydrogenase type-2, also known as HSD17B10, AB-binding alcohol dehydrogenase, or short chain type dehydrogenase/reductase XH98G2. This antibody has been extensively validated for western blotting using whole cell lysates.

ERAB Antibody with Control Lysate

Mouse anti human ERAB antibody recognizes 3-hydroxyacyl-CoA dehydrogenase type-2, also known as HSD17B10, AB-binding alcohol dehydrogenase, amyloid-beta peptide binding alcohol dehydrogenase, or endoplasmic reticulum-associated amyloid beta-peptide-binding protein.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI5E2
Isotype
IgG2a
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00400 WB 100 µl
VMA00400KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human ERAB antibody recognizes ERAB, also known as 3-hydroxyacyl-CoA dehydrogenase type-2, HSD17B10, 3-hydroxy-2-methylbutyryl-CoA dehydrogenase, AB-binding alcohol dehydrogenase, amyloid-beta peptide binding alcohol dehydrogenase, short chain dehydrogenase/reductase family 5C, member 1 and short chain type dehydrogenase/reductase XH98G2.

Encoded by the ERAB / HSD17B10 gene, 3-hydroxyacyl-CoA dehydrogenase type-2 is a member of the short-chain dehydrogenase/reductase superfamily. 3-hydroxyacyl-CoA dehydrogenase type-2 is mitochondrial protein that catalyzes the oxidation of a wide variety of fatty acids, alcohols, and steroids and has been implicated in the development of Alzheimer's disease. Mutations in the gene are the cause of 2-methyl-3-hydroxybutyryl-CoA dehydrogenase deficiency (MHBD). Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined (provided by RefSeq, Jul 2008).

Mouse anti Human ERAB antibody detects a band of 27 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti ERAB detects a band of approximately 27 kDa in HeLa cell lysates
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant human ERAB (HSD17B10) (NP_004484) produced in HEK293T cells
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HeLa lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
Q99714 Related reagents
Entrez Gene
HSD17B10 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0003857 3-hydroxyacyl-CoA dehydrogenase activity
GO:0005759 mitochondrial matrix
GO:0055114 oxidation-reduction process
GO:0006629 lipid metabolic process
GO:0008033 tRNA processing
GO:0008709 cholate 7-alpha-dehydrogenase activity
GO:0009083 branched chain family amino acid catabolic process
GO:0047015 3-hydroxy-2-methylbutyryl-CoA dehydrogenase activity
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of ERAB antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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