ERAB antibody | OTI5E2
Mouse anti ERAB
Mouse anti human ERAB antibody recognizes 3-hydroxyacyl-CoA dehydrogenase type-2, also known as HSD17B10, AB-binding alcohol dehydrogenase, or short chain type dehydrogenase/reductase XH98G2. This antibody has been extensively validated for western blotting using whole cell lysates.
ERAB Antibody with Control Lysate
Mouse anti human ERAB antibody recognizes 3-hydroxyacyl-CoA dehydrogenase type-2, also known as HSD17B10, AB-binding alcohol dehydrogenase, amyloid-beta peptide binding alcohol dehydrogenase, or endoplasmic reticulum-associated amyloid beta-peptide-binding protein.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- OTI5E2
- Isotype
- IgG2a
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00400 | WB | 100 µl | ||||
| VMA00400KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human ERAB antibody recognizes ERAB, also known as 3-hydroxyacyl-CoA dehydrogenase type-2, HSD17B10, 3-hydroxy-2-methylbutyryl-CoA dehydrogenase, AB-binding alcohol dehydrogenase, amyloid-beta peptide binding alcohol dehydrogenase, short chain dehydrogenase/reductase family 5C, member 1 and short chain type dehydrogenase/reductase XH98G2.
Encoded by the ERAB / HSD17B10 gene, 3-hydroxyacyl-CoA dehydrogenase type-2 is a member of the short-chain dehydrogenase/reductase superfamily. 3-hydroxyacyl-CoA dehydrogenase type-2 is mitochondrial protein that catalyzes the oxidation of a wide variety of fatty acids, alcohols, and steroids and has been implicated in the development of Alzheimer's disease. Mutations in the gene are the cause of 2-methyl-3-hydroxybutyryl-CoA dehydrogenase deficiency (MHBD). Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined (provided by RefSeq, Jul 2008).
Mouse anti Human ERAB antibody detects a band of 27 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Encoded by the ERAB / HSD17B10 gene, 3-hydroxyacyl-CoA dehydrogenase type-2 is a member of the short-chain dehydrogenase/reductase superfamily. 3-hydroxyacyl-CoA dehydrogenase type-2 is mitochondrial protein that catalyzes the oxidation of a wide variety of fatty acids, alcohols, and steroids and has been implicated in the development of Alzheimer's disease. Mutations in the gene are the cause of 2-methyl-3-hydroxybutyryl-CoA dehydrogenase deficiency (MHBD). Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined (provided by RefSeq, Jul 2008).
Mouse anti Human ERAB antibody detects a band of 27 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti ERAB detects a band of approximately 27 kDa in HeLa cell lysates
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography from ascites
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatography from ascites
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- Full length recombinant human ERAB (HSD17B10) (NP_004484) produced in HEK293T cells
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HeLa lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- Q99714 Related reagents
- Entrez Gene
- HSD17B10 Related reagents
- GO Terms
- GO:0005886 plasma membrane
- GO:0003857 3-hydroxyacyl-CoA dehydrogenase activity
- GO:0005759 mitochondrial matrix
- GO:0055114 oxidation-reduction process
- GO:0006629 lipid metabolic process
- GO:0008033 tRNA processing
- GO:0008709 cholate 7-alpha-dehydrogenase activity
- GO:0009083 branched chain family amino acid catabolic process
- GO:0047015 3-hydroxy-2-methylbutyryl-CoA dehydrogenase activity
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of ERAB antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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