Eplin antibody | OTI1A3

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Mouse anti Eplin

Product Type
PrecisionAb Monoclonal
Clone
OTI1A3
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00437
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human EPLIN antibody recognizes epithelial protein lost in neoplasm (EPLIN), also known as LIM domain and actin-binding protein 1, LIMA1 and sterol regulatory element binding protein 3.

Encoded by the EPLIN / LIMA1 gene, epithelial protein lost in neoplasm is a cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles. It is downregulated in some cancer cell lines. Alternatively spliced transcript variants encoding different isoforms have been described for LIMA1, and expression of some of the variants maybe independently regulated (provided by RefSeq, Aug 2011).

Mouse anti Human EPLIN antibody detects a band of 110 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti EPLIN detects a band of approximately 110 kDa in HEK293 cell lysates
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Recombinant protein fragment corresponding to aa 1-275 of human EPLIN or LIMA1 (NP_057441) produced in E.coli

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
Q9UHB6 Related reagents
Entrez Gene
LIMA1 Related reagents
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Eplin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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