Eplin antibody | OTI1A3

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Anti Eplin Antibody, clone OTI1A3 (PrecisionAb™ Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with EPLIN antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 77 second exposure. Arrow points to EPLIN (molecular weight 110 kDa)

Mouse anti Eplin

Mouse anti human EPLIN antibody recognizes LIM domain and actin-binding protein 1, also known as LIMA1, 1110021C24Rik, epithelial protein lost in neoplasm beta or sterol regulatory element binding protein 3. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type: PrecisionAb™ Monoclonal
Clone: OTI1A3
Isotype: IgG1
Format: Purified

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
VMA00437 Datasheet	WB MSDS	100 µl	Log in		Get Quote

Summary
Secondary Antibodies

Mouse anti Human EPLIN antibody recognizes epithelial protein lost in neoplasm (EPLIN), also known as LIM domain and actin-binding protein 1, LIMA1 and sterol regulatory element binding protein 3.

Encoded by the EPLIN / LIMA1 gene, epithelial protein lost in neoplasm is a cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles. It is downregulated in some cancer cell lines. Alternatively spliced transcript variants encoding different isoforms have been described for LIMA1, and expression of some of the variants maybe independently regulated (provided by RefSeq, Aug 2011).

Mouse anti Human EPLIN antibody detects a band of 110 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species: Human
Western Blotting: Anti EPLIN detects a band of approximately 110 kDa in HEK293 cell lysates
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography from ascites
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Immunogen: Recombinant protein fragment corresponding to aa 1-275 of human EPLIN or LIMA1 (NP_057441) produced in E.coli
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Guarantee: As supplied, 12 months from date of despatch.

More Information

UniProt: Q9UHB6 Related reagents
Entrez Gene: LIMA1 Related reagents
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of Eplin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in		Get Quote

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