Endothelial Cells antibody | PAL-E

100% Secure

Mouse anti Human Endothelial Cells (Vein Associated Antigen)

Product Type
Monoclonal Antibody
Clone
PAL-E
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
4109-0004 C IF 0.5 ml
Mouse anti Human endothlium antibody, clone PAL-E recognizes an antigen associated with venous endothelial cells.

Mouse anti Human endothlium antibody, clone PAL-E preferentially stains endothelial cells in medium and small veins, venules and capillaries in all organs. Negative, or weak staining is observed in large veins, arterioles and arteries. Lymphatic endothelium is also negative.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Goat
Rabbit
Pig
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Tissue Culture Supernatant - liquid
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Cell suspensions of freshly obtained human lymph node metastases of melanoma.
Fusion Partners
Spleen cells from immunized mice were fused with cells from theSP2/0 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of Endothelial Cells antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence 1/5 1/20
Immunohistology - Frozen 1/10 1/20
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Histology Positive Control Tissue
Endothelium.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F 0.1 mg F
Human anti Mouse IgG2a:HRP HCA037P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Product Specific References

Source Reference

  1. Schlingemann R.O. et al (1985) Monoclonal antibody PAL-E specific for endothelium.
    Lab Invest 52: 71-76

References for Endothelial Cells antibody

  1. Strohal, R. et al. (1991) Localized vs generalized pyogenic granuloma. A clinicopathologic study.
    Arch Dermatol. 127 (6): 856-61.
  2. Annessi, G. et al. (1992) Multinucleate cell angiohistiocytoma.
    Am J Dermatopathol. 14 (4): 340-4.
  3. Rappersberger K et al. (1990) Endemic Kaposi's sarcoma in human immunodeficiency virus type 1-seronegative persons: demonstration of retrovirus-like particles in cutaneous lesions.
    J Invest Dermatol. 95 (4): 371-81.