Endothelial Cells antibody | 1F10

100% Secure

Rat anti Human Endothelial Cells (Fully Differentiated)

Product Type
Monoclonal Antibody
Clone
1F10
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
4108-2108 C* 0.1 mg
Rat anti Human Endothelial cells antibody, clone 1F10 recognizes a ~150 kDa antigen present on fully differentiated continuous blood endothelial cells. Expression is low and inconsistent on sinusoidal endothelial cells of the liver and dermal lymphatic endothelia. Expression is absent on splenic sinusoidal endothelial cells (Goerdt et al. 1989).

Immunohistochemical studies indicate that the antigen recognized by Rat anti Human Endothelial cells antibody, clone 1F10 is strongly up-regulated in the muscularis propria of patients with Chron's disease, suggesting a possible role in the maintainence of chronic inflammation in this condition (Lügering et al. 1995).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - lyophilised
Reconstitution
With 0.5 ml sterile distilled water
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal, 1% Bovine Serum Albumin.
Immunogen
Cultured HUVECs.

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
Shelf Life
12 months from date of reconstitution.

More Information

Regulatory
For research purposes only

Applications of Endothelial Cells antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 1 1/50 1/200
Immunohistology - Paraffin
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human placenta

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F

Product Specific References

References for Endothelial Cells antibody

  1. Goerdt, S. et al. (1989) Characterization and differential expression of an endothelial cell-specific surface antigen in continuous and sinusoidal endothelial, in skin vascular lesions and in vitro.
    Exp Cell Biol. 57: 185-92.
  2. Goerdt, S. et al. (1991) Identification of a novel high molecular weight protein preferentially expressed by sinusoidal endothelial cells in normal human tissues.
    J Cell Biol. 113: 1425-37.
  3. Lügering, N. et al. (1995) Monoclonal antibody 1F10 immunoreactivity in inflammatory bowel disease: a new marker specific only for continuous endothelial cells.
    Eur J Gastroenterol Hepatol. 7: 777-81.
  4. Goerdt, S. et al. (1993) Inducible expression of MS-1 high-molecular-weight protein by endothelial cells of continuous origin and by dendritic cells/macrophages in vivo and in vitro.
    Am J Pathol. 142: 1409-22.
  5. Slowik, M.R. et al. (1993) Tumor necrosis factor activates human endothelial cells through the p55 tumor necrosis factor receptor but the p75 receptor contributes to activation at low tumor necrosis factor concentration.
    Am J Pathol. 143: 1724-30.