Rat anti Human Endothelial cells antibody, clone 1F10
recognizes a ~150 kDa antigen present on fully differentiated continuous blood endothelial cells. Expression is low and inconsistent on sinusoidal endothelial cells of the liver and dermal lymphatic endothelia. Expression is absent on splenic sinusoidal endothelial cells (Goerdt et al. 1989
Immunohistochemical studies indicate that the antigen recognized by Rat anti Human Endothelial cells antibody, clone 1F10 is strongly up-regulated in the muscularis propria of patients with Chron's disease, suggesting a possible role in the maintainence of chronic inflammation in this condition (Lügering et al. 1995
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - lyophilised
- With 0.5 ml sterile distilled water
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal, 1% Bovine Serum Albumin.
- Cultured HUVECs.
- Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
- 12 months from date of despatch
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Endothelial Cells antibody
|Immunohistology - Frozen 1
|Immunohistology - Paraffin
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Human placenta
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Secondary Antibodies Available
Product Specific References
References for Endothelial Cells antibody
Goerdt, S. et al. (1989) Characterization and differential expression of an endothelial cell-specific surface antigen in continuous and sinusoidal endothelial, in skin vascular lesions and in vitro.
Exp Cell Biol. 57: 185-92.
Goerdt, S. et al. (1991) Identification of a novel high molecular weight protein preferentially expressed by sinusoidal endothelial cells in normal human tissues.
J Cell Biol. 113: 1425-37.
Lügering, N. et al. (1995) Monoclonal antibody 1F10 immunoreactivity in inflammatory bowel disease: a new marker specific only for continuous endothelial cells.
Eur J Gastroenterol Hepatol. 7: 777-81.
Goerdt, S. et al. (1993) Inducible expression of MS-1 high-molecular-weight protein by endothelial cells of continuous origin and by dendritic cells/macrophages in vivo and in vitro.
Am J Pathol. 142: 1409-22.
Slowik, M.R. et al. (1993) Tumor necrosis factor activates human endothelial cells through the p55 tumor necrosis factor receptor but the p75 receptor contributes to activation at low tumor necrosis factor concentration.
Am J Pathol. 143: 1724-30.
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