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EMR1 antibody | A10

Hamster anti Human EMR1:Alexa Fluor® 647

Product Type
Monoclonal Antibody
Clone
A10
Isotype
IgG
Specificity
EMR1

Product Code Applications Pack Size List Price Your Price Qty
MCA2674A647
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests/1ml loader
List Price Your Price
loader

Hamster anti Human EMR1 antibody, clone A10 recognizes human EMR1 (EGF-like module containing, mucin-like, hormone receptor-like 1). EMR1 is a seven-span transmembrane protein which is a member of the EGF-TM7 family of receptors. EMR1 is the human ortholog of murine F4/80 but unlike F4/80, the expression of EMR1 in humans is restricted to eosinophils and is not expressed on macrophage populations. The detailed structure and function of EMR1 is still unknown.

Target Species
Human
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
1%Bovine Serum Albumin
Immunogen
Fibroblast cell line ARH102 expressing CD97-EMR1 (EGF1-6)
Approx. Protein Concentrations
IgG concentration 0.05mg/ml
Fusion Partners
Spleen cells from immunized Armenian hamster were fused with cells of the mouse SP2/0 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Alexa Fluor®647 650 665
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Description Product Code Applications Pack Size List Price Your Price Quantity
Hamster (Armenian) IgG Negative Control:Alexa Fluor® 647 MCA2356A647 F 100 Tests/1ml loader
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List Price Your Price
loader
Description Hamster (Armenian) IgG Negative Control:Alexa Fluor® 647

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock

Source Reference

  1. Hamann, J. et al. (2007) EMR1, the human homolog of F4/80, is an eosinophil-specific receptor.
    Eur J Immunol. 37 (10): 2797-802.

References for EMR1 antibody

  1. van Eijk, M. et al. (2010) Differential expression of the EGF-TM7 family members CD97 and EMR2 in lipid-laden macrophages in atherosclerosis, multiple sclerosis and Gaucher disease.
    Immunol Lett. 129 (2): 64-71.
  2. Pothoven, K.L. et al. (2017) Neutrophils are a major source of the epithelial barrier disrupting cytokine oncostatin M in patients with mucosal airways disease.
    J Allergy Clin Immunol. 139 (6): 1966-78.e9.
  3. Fuchs, A. et al. (2021) Associations Among Adipose Tissue Immunology, Inflammation, Exosomes and Insulin Sensitivity in People With Obesity and Nonalcoholic Fatty Liver Disease.
    Gastroenterology. 161 (3): 968-981.e12.

RRID
AB_10845950
UniProt
Q14246
Entrez Gene
EMR1
GO Terms
GO:0005509 calcium ion binding
GO:0007155 cell adhesion
GO:0004930 G-protein coupled receptor activity
GO:0005887 integral to plasma membrane
GO:0007218 neuropeptide signaling pathway

MCA2674A647

1511 154029

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