EMD antibody | AB01/4H6

100% Secure


Mouse anti EMD

Product Type
PrecisionAb Monoclonal
Clone
AB01/4H6
Isotype
IgG2a
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00853
Datasheet
IP WB
SDS
100 µl loader

Mouse anti EMD antibody recognizes emerin, also known as EDMD, STA.

EMD is a ubiquitous inner nuclear membrane protein that contributes to genome organization, regulation of gene expression, and mechanical stress signaling (Essawy et al. 2019). However, the protein can also be found in outer membranes during interphase within mitosis (Dubińska-Magiera et al. 2019). Deregulation of EMD can play a role in malignant transformation, and the localization of emerin can be used to identify tumor cells that have nuclear shape instability (Reis-Sobreiro et al. 2018). Mutations in the EMD gene, most often leading to an absence of EMD, cause X-linked Emery-Dreifuss muscular dystrophy (X-EDMD) (Essawy et al. 2019). This disease is characterized by contractures of the elbow and Achilles tendons, progressive muscle wasting and cardiac conduction defects (Dubińska-Magiera et al. 2019).

Product Details

Target Species
Human
Western Blotting
Mouse anti EMD antibody detects a band of approximately 31 kDa in HEK293 cell lysates
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-254 of human EMD
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P50402
Entrez Gene
EMD
GO Terms
GO:0006936 muscle contraction
GO:0003779 actin binding
GO:0016021 integral to membrane
GO:0005783 endoplasmic reticulum
GO:0005637 nuclear inner membrane
GO:0005640 nuclear outer membrane
GO:0005874 microtubule
GO:0007517 muscle organ development
GO:0035414 negative regulation of catenin import into nucleus
GO:0046827 positive regulation of protein export from nucleus
GO:0048147 negative regulation of fibroblast proliferation
GO:0048487 beta-tubulin binding
GO:0060828 regulation of canonical Wnt receptor signaling pathway
GO:0071363 cellular response to growth factor stimulus
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of EMD antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

Fluorescent Spectraviewer

Watch the Tool Tutorial Video ▸

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra