EMD antibody | AB01/4H6
Mouse anti EMD antibody recognizes emerin, also known as EDMD, STA.
EMD is a ubiquitous inner nuclear membrane protein that contributes to genome organization, regulation of gene expression, and mechanical stress signaling (Essawy et al. 2019). However, the protein can also be found in outer membranes during interphase within mitosis (Dubińska-Magiera et al. 2019). Deregulation of EMD can play a role in malignant transformation, and the localization of emerin can be used to identify tumor cells that have nuclear shape instability (Reis-Sobreiro et al. 2018). Mutations in the EMD gene, most often leading to an absence of EMD, cause X-linked Emery-Dreifuss muscular dystrophy (X-EDMD) (Essawy et al. 2019). This disease is characterized by contractures of the elbow and Achilles tendons, progressive muscle wasting and cardiac conduction defects (Dubińska-Magiera et al. 2019).
EMD is a ubiquitous inner nuclear membrane protein that contributes to genome organization, regulation of gene expression, and mechanical stress signaling (Essawy et al. 2019). However, the protein can also be found in outer membranes during interphase within mitosis (Dubińska-Magiera et al. 2019). Deregulation of EMD can play a role in malignant transformation, and the localization of emerin can be used to identify tumor cells that have nuclear shape instability (Reis-Sobreiro et al. 2018). Mutations in the EMD gene, most often leading to an absence of EMD, cause X-linked Emery-Dreifuss muscular dystrophy (X-EDMD) (Essawy et al. 2019). This disease is characterized by contractures of the elbow and Achilles tendons, progressive muscle wasting and cardiac conduction defects (Dubińska-Magiera et al. 2019).
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti EMD antibody detects a band of approximately 31 kDa in HEK293 cell lysates
- Product Form
- Purified IgG - Liquid
- Preparation
- Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
- Immunogen
- E. coli-derived recombinant protein of amino acids 1-254 of human EMD
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P50402
- Entrez Gene
- EMD
- GO Terms
- GO:0006936 muscle contraction
- GO:0003779 actin binding
- GO:0016021 integral to membrane
- GO:0005783 endoplasmic reticulum
- GO:0005637 nuclear inner membrane
- GO:0005640 nuclear outer membrane
- GO:0005874 microtubule
- GO:0007517 muscle organ development
- GO:0035414 negative regulation of catenin import into nucleus
- GO:0046827 positive regulation of protein export from nucleus
- GO:0048147 negative regulation of fibroblast proliferation
- GO:0048487 beta-tubulin binding
- GO:0060828 regulation of canonical Wnt receptor signaling pathway
- GO:0071363 cellular response to growth factor stimulus
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of EMD antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunoprecipitation | |||
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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|---|---|---|---|---|---|
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