ELF3 antibody | OTI4G5
Mouse anti ELF3
ELF3 antibody recognizes the ETS-related transcription factor Elf-3, also known as E74-like factor 3 (ets domain transcription factor), epithelial-restricted with serine box, epithelium-restricted Ets protein ESX or epithelium-specific Ets transcription factor 1.
ELF3 Antibody with Control Lysate
ELF3 antibody recognizes the ETS-related transcription factor Elf-3, also known as E74-like factor 3 (ets domain transcription factor), epithelial-restricted with serine box, epithelium-restricted Ets protein ESX or epithelium-specific Ets transcription factor 1.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- OTI4G5
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00602 | WB | 100 µl | ||||
| VMA00602KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human ELF3 antibody recognizes the ETS-related transcription factor Elf-3, also known as E74-like factor 3 (ets domain transcription factor), epithelial-restricted with serine box, epithelium-restricted Ets protein ESX and epithelium-specific Ets transcription factor 1.
Mouse anti Human ELF3 antibody detects a band of 47 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Mouse anti Human ELF3 antibody detects a band of 47 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti ELF3 antibody detects a band of approximately 47 kDa in A431 cell lysates
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
<50% Glycerol - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
<50% Glycerol - Immunogen
- E. coli-derived recombinant human ELF3 (aa 175-286)
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg A431 lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- P78545 Related reagents
- Entrez Gene
- ELF3 Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0003713 transcription coactivator activity
- GO:0005634 nucleus
- GO:0005794 Golgi apparatus
- GO:0006366 transcription from RNA polymerase II promoter
- GO:0006954 inflammatory response
- GO:0008544 epidermis development
- GO:0016563 transcription activator activity
- GO:0016564 transcription repressor activity
- GO:0030855 epithelial cell differentiation
- GO:0043565 sequence-specific DNA binding
- GO:0060056 mammary gland involution
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of ELF3 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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