EIF4B antibody

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Mouse anti EIF4B

Product Type
PrecisionAb Monoclonal
Isotype
IgG2b
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00120
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human EIF4B antibody recognizes EIF4B, also known as eukaryotic translation initiation factor 4B.

Eukaryotic translation initiation factor 4B is necessary for the binding of mRNA to ribosomes, promoting the ATPase activity and ATP-dependent RNA unwinding activity of the related proteins, EIF4A and EIF4F.

Mouse anti Human EIF4B antibody detects a band of 80 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti EIF4B detects a band of approximately 80 kDa in K562 cell lysates.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Purified recombinant fragment of human EIF4B expressed in E. coli

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P23588 Related reagents
Entrez Gene
EIF4B Related reagents
GO Terms
GO:0000166 nucleotide binding
GO:0000289 nuclear-transcribed mRNA poly(A) tail shortening
GO:0003743 translation initiation factor activity
GO:0005829 cytosol
GO:0006446 regulation of translational initiation
GO:0008286 insulin receptor signaling pathway
GO:0016281 eukaryotic translation initiation factor 4F complex
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of EIF4B antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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