EIF2AK2 antibody

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Mouse anti EIF2AK2

EIF2AK2 antibody recognizes interferon-induced, double-stranded RNA-activated protein kinase, also known as P1/eIF-2A protein kinase, eIF-2A protein kinase 2, interferon-inducible elF2alpha kinase, p68 kinase, or protein phosphatase 1.

EIF2AK2 Antibody with Control Lysate

EIF2AK2 antibody recognizes interferon-induced, double-stranded RNA-activated protein kinase, also known as P1/eIF-2A protein kinase, eIF-2A protein kinase 2, interferon-inducible elF2alpha kinase, p68 kinase, or protein phosphatase 1.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00312 WB 100 µl
VMA00312KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human EIF2AK2 antibody recognizes the interferon-induced, double-stranded RNA-activated protein kinase, also known as P1/eIF-2A protein kinase, eIF-2A protein kinase 2, interferon-induced, interferon-inducible elF2alpha kinase, p68 kinase, protein kinase, interferon-inducible double stranded RNA dependent, protein phosphatase 1, regulatory subunit 83 and tyrosine-protein kinase EIF2AK2.

Encoded by the EIF2AK2 gene, interferon-induced, double-stranded RNA-activated protein kinase is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. Three transcript variants encoding two different isoforms have been found for EIF2AK2 (provided by RefSeq, Oct 2011).

Mouse anti Human EIF2AK2 antibody detects a band of 74 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti EIF2AK2 detects a band of approximately 74 kDa in HEK293 cell lysates
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
Preparation
20μl Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant EIF2AK2
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HEK293 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P19525 Related reagents
Entrez Gene
EIF2AK2 Related reagents
GO Terms
GO:0005515 protein binding
GO:0003725 double-stranded RNA binding
GO:0009615 response to virus
GO:0005829 cytosol
GO:0004694 eukaryotic translation initiation factor 2alpha kinase activity
GO:0005524 ATP binding
GO:0008601 protein phosphatase type 2A regulator activity
GO:0019054 modulation by virus of host cellular process
GO:0019058 viral infectious cycle
GO:0030683 evasion by virus of host immune response
GO:0033689 negative regulation of osteoblast proliferation
GO:0046777 protein autophosphorylation
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of EIF2AK2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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