EIF2A antibody | CD02/2H5

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Anti EIF2A Antibody, clone CD02/2H5 (PrecisionAb™ Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with EIF2A antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 77 second exposure. Arrow points to EIF2A (molecular weight 62 kDa).

Mouse anti EIF2A

EIF2A antibody recognizes eukaryotic translation initiation factor 2A.

Product Type: PrecisionAb™ Monoclonal
Clone: CD02/2H5
Isotype: IgG2a
Format: Purified

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
VMA00644	WB			100 µl

Summary
Secondary Antibodies

Mouse anti Human EIF2A antibody recognizes eukaryotic translation initiation factor 2A.

EIF2A is a 65 kDa protein that catalyzes the formation of puromycin-sensitive 80S preinitiation complexes (Zoll et al., 2002 (PubMed 12133843)) (supplied by OMIM, Mar 2008).

Mouse anti Human EIF2A antibody detects a band of 62 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species

Human

Western Blotting

Mouse anti EIF2A antibody detects a band of approximately 62 kDa in Jurkat cell lysates

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse
Rat

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Preparation

Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)

Immunogen

E. coli-derived recombinant human EIF2A (aa 448-576)

Instructions For Use

Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles
Guarantee: As supplied, 12 months from date of despatch

More Information

UniProt: Q9BY44 Related reagents
Entrez Gene: EIF2A Related reagents
GO Terms: GO:0000049 tRNA binding; GO:0003743 translation initiation factor activity; GO:0005850 eukaryotic translation initiation factor 2 complex; GO:0006417 regulation of translation; GO:0042255 ribosome assembly; GO:0043022 ribosome binding
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory: For research purposes only

Applications of EIF2A antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB

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