EIF2A antibody | CD02/2H5
Mouse anti EIF2A
EIF2A antibody recognizes eukaryotic translation initiation factor 2A.
EIF2A Antibody with Control Lysate
EIF2A antibody recognizes eukaryotic translation initiation factor 2A.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- CD02/2H5
- Isotype
- IgG2a
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00644 | WB | 100 µl | ||||
| VMA00644KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human EIF2A antibody recognizes eukaryotic translation initiation factor 2A.
EIF2A is a 65 kDa protein that catalyzes the formation of puromycin-sensitive 80S preinitiation complexes (Zoll et al., 2002 (PubMed 12133843)) (supplied by OMIM, Mar 2008).
Mouse anti Human EIF2A antibody detects a band of 62 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
EIF2A is a 65 kDa protein that catalyzes the formation of puromycin-sensitive 80S preinitiation complexes (Zoll et al., 2002 (PubMed 12133843)) (supplied by OMIM, Mar 2008).
Mouse anti Human EIF2A antibody detects a band of 62 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti EIF2A antibody detects a band of approximately 62 kDa in Jurkat cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- E. coli-derived recombinant human EIF2A (aa 448-576)
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg Jurkat lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- Q9BY44 Related reagents
- Entrez Gene
- EIF2A Related reagents
- GO Terms
- GO:0000049 tRNA binding
- GO:0003743 translation initiation factor activity
- GO:0005850 eukaryotic translation initiation factor 2 complex
- GO:0006417 regulation of translation
- GO:0042255 ribosome assembly
- GO:0043022 ribosome binding
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of EIF2A antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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