EGF R (pTyr1173) antibody
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Mouse anti EGF Receptor (pTyr1173)
EGF receptor (pTyr1173) antibody recognizes the epidermal growth factor receptor, also known as proto-oncogene c-ErbB-1 phosphorylated at Tyr1173.
- Product Type
- PrecisionAb™ Monoclonal
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00752 | WB | 100 µl |
Mouse anti EGF receptor antibody recognizes the epidermal growth factor receptor, also known as proto-oncogene c-ErbB-1 or receptor tyrosine-protein kinase erbB-1, when phosphorylated at threonine 1173. EGF R plays a role in numerous cellular processes such as cell proliferation and apoptosis through the activation of downstream signaling pathways (Scaltriti and Baselga 2006). EGF
R is autophosphorylated at tyrosine 1173 upon ligand binding (Downward et al. 1985). PRMT5- mediated EGF R arginine 1175 methylation modulates EGF R autophoshorylation on tyrosine 1173 (Hsu et al. 2011). EGF R phosphorylation on tyrosine 1173 correlates with a poor prognosis in oral squamous cell carcinomas (OSCC) (Monteiro et al. 2014).
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti EGF receptor (pTyr1173) detects a band of approximately 175 kDa in A431 pervanadate-treated A431 (treated lysate) cell lysates
- Product Form
- Purified IgG - Liquid
- Preparation
- Mouse monoclonal antibody purified by thiophillic adsorption and size exclusion chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
PEG & Sucrose - Immunogen
- Phosphopeptide conjugate to KLH
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- P00533 Related reagents
- Entrez Gene
- EGFR Related reagents
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of EGF R (pTyr1173) antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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