EGF R (pTyr1173) antibody

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Anti EGF Receptor (pTyr1173) Antibody (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti EGF Receptor (pTyr1173) Antibody (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti EGF Receptor (pTyr1173) Antibody (PrecisionAb Monoclonal Antibody) gallery image 1 — Western Blot analysis of A431 untreated and pervanadate (PVD) treated whole cell lysates probed with (A) Mouse anti EGF R antibody (VMA00061) or (B) Mouse anti EGF R (pTyr1173) antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P). Membranes were treated with (+) and without (-) lambda protein phosphatase and visualized on the ChemiDoc MP with a (A) 2 second or a (B) 11 second exposure. EGF R (pTyr1173) has a molecular weight of 175 kDa.

Anti EGF Receptor (pTyr1173) Antibody (PrecisionAb Monoclonal Antibody) gallery image 2 — Normalized signal intensity of EGF R (pTyr1173) from PVD treated and untreated lysates, treated with (+) or without (-) lambda protein phosphatase. Lambda protein phosphatase dephosphorylates serine, threonine and tyrosine residues. Normalized signal intensity was calculated by dividing the TPN-Normalized signal intensity for each phosphoprotein by the TPN-normalized intensity of the corresponding total protein.
Learn more about TPN Total Protein Normalization).

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Mouse anti EGF Receptor (pTyr1173)

Product Type: PrecisionAb Monoclonal
Isotype: IgG1
Format: Purified
Specificity: EGF R (pTyr1173)

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Mouse anti EGF receptor antibody recognizes the epidermal growth factor receptor, also known as proto-oncogene c-ErbB-1 or receptor tyrosine-protein kinase erbB-1, when phosphorylated at threonine 1173. EGF R plays a role in numerous cellular processes such as cell proliferation and apoptosis through the activation of downstream signaling pathways (Scaltriti and Baselga 2006). EGF R is autophosphorylated at tyrosine 1173 upon ligand binding (Downward et al. 1985). PRMT5- mediated EGF R arginine 1175 methylation modulates EGF R autophoshorylation on tyrosine 1173 (Hsu et al. 2011). EGF R phosphorylation on tyrosine 1173 correlates with a poor prognosis in oral squamous cell carcinomas (OSCC) (Monteiro et al. 2014).

Target Species: Human
Western Blotting: Mouse anti EGF receptor (pTyr1173) detects a band of approximately 175 kDa in A431 pervanadate-treated A431 (treated lysate) cell lysates
Product Form: Purified IgG - Liquid
Preparation: Mouse monoclonal antibody purified by thiophillic adsorption and size exclusion chromatography from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide
PEG & Sucrose
Immunogen: Phosphopeptide conjugate to KLH
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.