EGF R (pTyr1148) antibody

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Mouse anti EGF Receptor (pTyr1148)

EGF receptor (pTyr1148) antibody recognizes the epidermal growth factor receptor, also known as proto-oncogene c-ErbB-1 phosphorylated at Tyr1148.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG3
Format
Purified
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00751 WB 100 µl
Mouse anti EGF receptor antibody recognizes the epidermal growth factor receptor, also known as proto-oncogene c-ErbB-1 or receptor tyrosine-protein kinase erbB-1, when phosphorylated at threonine 1148. EGF R plays a role in numerous cellular processes such as cell proliferation and apoptosis through the activation of downstream signaling pathways (Scaltriti and Baselga 2006).EGF R is autophosphorylated at tyrosine 1148 upon ligand binding (Downward et al. 1985). Phosphorylation at 1148 serves as a docking site for the adaptor protein Shc (Okutani et al. 1994). Binding of Shc to EGF R regulates association of the Grb2-Sos complex and is important for Ras signaling pathway activation (Sakaguchi et al. 1998).

Product Details

Target Species
Human
Western Blotting
Mouse anti EGF receptor (pTyr1148) detects a band of approximately 175 kDa in pervanadate-treated A431 (treated lysate) cell lysates
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody purified by ultrafiltration and size exclusion chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
PEG & Sucrose
Immunogen
Phosphopeptide conjugated to hemocyanin
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
As supplied, 12 months from date of despatch

More Information

UniProt
P00533 Related reagents
Entrez Gene
EGFR Related reagents
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of EGF R (pTyr1148) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
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