EGF R (pTyr1086) antibody
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Mouse anti EGF Receptor (pTyr1086)
EGF receptor (pTyr1086) antibody recognizes the epidermal growth factor receptor, also known as proto-oncogene c-ErbB-1 phosphorylated at Tyr1086.
- Product Type
- PrecisionAb™ Monoclonal
- Isotype
- IgG2a
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00750 | WB | 100 µl |
Mouse anti EGF receptor antibody recognizes the epidermal growth factor receptor, also known as proto-oncogene c-ErbB-1 or receptor tyrosine-protein kinase erbB-1, when phosphorylated at threonine 1086. EGF R plays a role in numerous cellular processes such as cell proliferation and apoptosis through the activation of downstream signaling pathways (Scaltriti and Baselga 2006). EGF
receptor is autophosphorylated at tyrosine 1086 upon ligand binding (Hsuan et al. 1989). This phosphorylation is critical for kinase loop activation (Kovacs et al. 2015). Macrophages have been reported to stimulate gastric and colorectal cancer cell invasion by activation of EGF R phosphorylation at tyrosine 1086 (Cardoso et al. 2014).
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti EGF receptor (pTyr1086) detects a band of approximately 175 kDa in pervanadate-treated A431 (treated lysate) cell lysates
- Product Form
- Purified IgG - Liquid
- Preparation
- Mouse monoclonal antibody purified by ultrafiltration and size exclusion chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
PEG & Sucrose - Immunogen
- Phosphopeptide conjugated to hemocyanin
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- P00533 Related reagents
- Entrez Gene
- EGFR Related reagents
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of EGF R (pTyr1086) antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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