EGF R (pTyr1045) antibody | AbD30783

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Human anti EGF R (pTyr1045)

Product Type
PrecisionAb Monoclonal
Clone
AbD30783
Isotype
HuCAL Fab bivalent
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00515
Datasheet
WB
MSDS
100 µl loader

Human anti EGF R (pTyr1045) antibody recognizes the epidermal growth factor receptor (EGF R), also known as proto-oncogene c-ErbB-1 or receptor tyrosine-protein kinase erbB-1, when phosphorylated at tyrosine 1045. EGF R is a transmembrane glycoprotein and a member of the ErbB receptor tyrosine kinase family. EGF R plays a role in numerous cellular processes such as cell proliferation and apoptosis through the activation of downstream signaling pathways (Scaltriti, M. & Baselga, J. 2006). The E3 ubiquitin ligase, Cbl, binds to tyrosine 1045 upon its phosphorylation (Levkowitz, G. et al. 1999) and mediates its monoubiquitination, which results in receptor endocytosis followed by endosomal/lysosomal sorting (Grøvdal, L.M. et al. 2004 and Haglund, K. & Dikic, I. 2012).

Product Details

Target Species
Human
Western Blotting
Human anti EGF R (pTyr1045) detects a band of approximately 179 kDa in MDA-MB-468 + EGF (treated lysate) cell lysates
Product Form
A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via an inactive variant of the bacterial alkaline phosphatase (BAP) fusion protein. The antibody is tagged with DYKDDDDK and His (HHHHHH) tags.
Preparation
Prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Phosphorylated EGF R peptide conjugated to BSA and human transferrin
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P00533
Entrez Gene
EGFR
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
His-tag is a registered trademark of EMD Biosciences.
Regulatory
For research purposes only

Applications of EGF R (pTyr1045) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/200 1/2000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.