EGF R (pTyr1045) antibody | AbD30783

Human anti EGF R (pTyr1045)

Product Type
PrecisionAb Monoclonal
Clone
AbD30783
Isotype
HuCAL Fab bivalent
Format
Purified
Specificity
EGF R (pTyr1045)

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VMA00515
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Human anti EGF R (pTyr1045) antibody recognizes the epidermal growth factor receptor (EGF R), also known as proto-oncogene c-ErbB-1 or receptor tyrosine-protein kinase erbB-1, when phosphorylated at tyrosine 1045. EGF R is a transmembrane glycoprotein and a member of the ErbB receptor tyrosine kinase family. EGF R plays a role in numerous cellular processes such as cell proliferation and apoptosis through the activation of downstream signaling pathways (Scaltriti, M. & Baselga, J. 2006). The E3 ubiquitin ligase, Cbl, binds to tyrosine 1045 upon its phosphorylation (Levkowitz, G. et al. 1999) and mediates its monoubiquitination, which results in receptor endocytosis followed by endosomal/lysosomal sorting (Grøvdal, L.M. et al. 2004 and Haglund, K. & Dikic, I. 2012). PrecisionAb Antibodies

Target Species
Human
Western Blotting
Human anti EGF R (pTyr1045) detects a band of approximately 179 kDa in MDA-MB-468 + EGF (treated lysate) cell lysates.
Product Form
A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via an inactive variant of the bacterial alkaline phosphatase (BAP) fusion protein. The antibody is tagged with DYKDDDDK and His (HHHHHH) tags.
Preparation
Prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.01% Thiomersal.
Immunogen
Phosphorylated EGF R peptide conjugated to BSA and human transferrin
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/200 1/2000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Synonyms
ERBB1
UniProt
P00533
Entrez Gene
EGFR
View more products with EGF R specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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