EGF R (pSer1047) antibody
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Mouse anti EGF Receptor (pSer1047)
EGF receptor (pSer1047) antibody recognizes the epidermal growth factor receptor, also known as proto-oncogene c-ErbB-1 phosphorylated at Ser1047.
- Product Type
- PrecisionAb™ Monoclonal
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| VMA00746 | WB | 100 µl |
|
Mouse anti EGF receptor antibody recognizes the epidermal growth factor receptor, also known as proto-oncogene c-ErbB-1 or receptor tyrosine-protein kinase erbB-1, when phosphorylated at serine 1047. EGF R is a cell surface receptor for members of the epidermal growth factor family. EGF R plays a role in numerous cellular processes such as cell proliferation and apoptosis through the activation of downstream signaling pathways (Scaltriti and Baselga 2006). Serine 1047 is phosphorylated by calmodulin-dependent protein kinase II (CaM kinase II) (Feinmesser et al. 1999). The phosphorylation of serine 1047 is important for EGF receptor desensitization (Countaway et al.
1992).
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti EGF receptor (pSer1047) detects a band of approximately 175 kDa in pervanadate-treated A431 (treated lysate) cell lysates
- Product Form
- Purified IgG - Liquid
- Preparation
- Mouse monoclonal antibody purified by thiophillic adsorption and size exclusion chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
PEG & Sucrose - Immunogen
- Phosphopeptide conjugated to KLH
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Guarantee
- As supplied, 12 months from date of despatch
More Information
- UniProt
- P00533 Related reagents
- Entrez Gene
- EGFR Related reagents
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of EGF R (pSer1047) antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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