EGF R (pSer1047) antibody

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Anti EGF Receptor (pSer1047) Antibody (PrecisionAb™ Monoclonal Antibody) thumbnail image 1

Anti EGF Receptor (pSer1047) Antibody (PrecisionAb™ Monoclonal Antibody) thumbnail image 2

Anti EGF Receptor (pSer1047) Antibody (PrecisionAb™ Monoclonal Antibody) gallery image 1 — Western Blot analysis of A431 untreated and pervanadate (PVD) treated whole cell lysates probed with (A) Mouse anti EGF R antibody (VMA00061) or (B) Mouse anti EGF R (pSer1047) antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P). Membranes were treated with (+) and without (-) lambda protein phosphatase and visualized on the ChemiDoc MP with a (A) 2 second or a (B) 5 second exposure. EGF R (pSer1047) has a molecular weight of 175 kDa.

Anti EGF Receptor (pSer1047) Antibody (PrecisionAb™ Monoclonal Antibody) gallery image 2 — Normalized signal intensity of EGF R (pSer1047) from PVD treated and untreated lysates, treated with (+) or without (-) lambda protein phosphatase. Lambda protein phosphatase dephosphorylates serine, threonine and tyrosine residues. Normalized signal intensity was calculated by dividing the TPN-Normalized signal intensity for each phosphoprotein by the TPN-normalized intensity of the corresponding total protein.
Learn more about TPN Total Protein Normalization).

Mouse anti EGF Receptor (pSer1047)

EGF receptor (pSer1047) antibody recognizes the epidermal growth factor receptor, also known as proto-oncogene c-ErbB-1 phosphorylated at Ser1047.

Product Type: PrecisionAb™ Monoclonal
Isotype: IgG1
Format: Purified

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
VMA00746	WB			100 µl

Summary
Secondary Antibodies

Mouse anti EGF receptor antibody recognizes the epidermal growth factor receptor, also known as proto-oncogene c-ErbB-1 or receptor tyrosine-protein kinase erbB-1, when phosphorylated at serine 1047. EGF R is a cell surface receptor for members of the epidermal growth factor family. EGF R plays a role in numerous cellular processes such as cell proliferation and apoptosis through the activation of downstream signaling pathways (Scaltriti and Baselga 2006). Serine 1047 is phosphorylated by calmodulin-dependent protein kinase II (CaM kinase II) (Feinmesser et al. 1999). The phosphorylation of serine 1047 is important for EGF receptor desensitization (Countaway et al. 1992).

Product Details

Target Species: Human
Western Blotting: Mouse anti EGF receptor (pSer1047) detects a band of approximately 175 kDa in pervanadate-treated A431 (treated lysate) cell lysates
Product Form: Purified IgG - Liquid
Preparation: Mouse monoclonal antibody purified by thiophillic adsorption and size exclusion chromatography from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide
PEG & Sucrose
Immunogen: Phosphopeptide conjugated to KLH
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage: Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Shelf Life: As supplied, 12 months from date of despatch

More Information

UniProt: P00533 Related reagents
Entrez Gene: EGFR Related reagents
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory: For research purposes only

Applications of EGF R (pSer1047) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB

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