EGF R (L858R Mutant) antibody | RM380

Rabbit anti EGF R (L858R Mutant)

Product Type
Monoclonal Antibody
Clone
RM380
Isotype
IgG
Specificity
EGF R (L858R Mutant)

Product Code Applications Pack Size List Price Your Price Qty
MCA6370
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SDS Safety Datasheet SDS
P WB 0.1 ml loader
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Rabbit anti EGF R (L858R mutant) antibody recognizes epidermal growth factor receptor (EGF R), also known as ERBB1 and HER1, mutated at the residue L858R.

EGF R is a member of the ErbB family of receptor tyrosine kinases (RTKs) involved with activation of multiple signaling pathways including Ras/MAPK, PI3K/AKT, and PKC signaling (Sigismund et al. 2018). The EGF R plasma membrane receptor achieves this effect by binding to a range of extracellular growth factors, undergoing a conformational change, and transducing intracellular signaling (Liu et al. 2017). EGF R was one of the first identified oncogenes, and overexpression of EGF R has been reported in many human malignancies. The EGF R inhibitors gefitinib and erlotinib are used for treatment of advanced non-small cell lung cancer (NSCLC) (Bethune et al. 2010). The L858R activating mutation is a single-point substitution mutation in EGF R and is common in NSCLC. It is associated with decreased sensitivity to gefitinib and erlotinib (Gazdar, 2010).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from animal origin-free culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
50% Glycerol
Immunogen
A peptide corresponding to the the EGF R L858R mutant
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1/100 1/200
Western Blotting 1/1000 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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UniProt
P00533
Entrez Gene
EGFR
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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