EGF R antibody

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Mouse anti EGF Receptor

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG2a
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00061
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human EGF receptor antibody recognizes the epidermal growth factor receptor, also known as Proto-oncogene c-ErbB-1 or receptor tyrosine-protein kinase erbB-1.

The protein encoded by EGFR is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGF receptor is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in EGFR are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for EGFR. (provided by RefSeq, Jul 2010).

Mouse anti Human EGF receptor antibody detects a band of 170 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti EGF R detects a band of approximately 170 kDa in A431 cell lysates.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from ascites.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
E. coli derived recombinant protein corresponding to aa 363-499 of the human EGF receptor

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P00533 Related reagents
Entrez Gene
EGFR Related reagents
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of EGF R antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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